論文 - 詳細
| RRC ID | 42600 |
|---|---|
| 著者 | Arasaki K, Taniguchi M, Tani K, Tagaya M. |
| タイトル | RINT-1 regulates the localization and entry of ZW10 to the syntaxin 18 complex. |
| ジャーナル | Mol Biol Cell |
| Abstract |
RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. We have recently reported that RINT-1, together with the dynamitin-interacting protein ZW10 and others, is associated with syntaxin 18, an endoplasmic reticulum (ER)-localized SNARE involved in membrane trafficking between the ER and Golgi. To address the role of RINT-1 in membrane trafficking, we examined the effects of overexpression and knockdown of RINT-1 on Golgi morphology and protein transport from the ER. Overexpression of the N-terminal region of RINT-1, which is responsible for the interaction with ZW10, caused redistribution of ZW10. Concomitantly, ER-to-Golgi transport was blocked and the Golgi was dispersed. Knockdown of RINT-1 also disrupted membrane trafficking between the ER and Golgi. Notably, silencing of RINT-1 resulted in a reduction in the amount of ZW10 associated with syntaxin 18, concomitant with ZW10 redistribution. In contrast, no redistribution or release of RINT-1 from the syntaxin 18 complex was observed when ZW10 expression was reduced. These results taken together suggest that RINT-1 coordinates the localization and function of ZW10 by serving as a link between ZW10 and the SNARE complex comprising syntaxin 18. |
| 巻・号 | 17(6) |
| ページ | 2780-8 |
| 公開日 | 2006-6-1 |
| DOI | 10.1091/mbc.e05-10-0973 |
| PII | E05-10-0973 |
| PMID | 16571679 |
| PMC | PMC1474792 |
| MeSH | Brefeldin A / pharmacology Cell Cycle Cell Cycle Proteins / metabolism* Cell Membrane / metabolism Endoplasmic Reticulum / metabolism Golgi Apparatus / metabolism HeLa Cells Humans Intracellular Signaling Peptides and Proteins / deficiency Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Nuclear Proteins / deficiency Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding Protein Transport Qa-SNARE Proteins / metabolism* RNA Interference Recombinant Fusion Proteins / metabolism SNARE Proteins / metabolism* Transfection Zinc Fingers |
| IF | 3.791 |
| 引用数 | 56 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |