| RRC ID |
42607
|
| 著者 |
Ogawa M, Yanoma S, Nagashima Y, Okamoto N, Ishikawa H, Haruki A, Miyagi E, Takahashi T, Hirahara F, Miyagi Y.
|
| タイトル |
Paradoxical discrepancy between the serum level and the placental intensity of PP5/TFPI-2 in preeclampsia and/or intrauterine growth restriction: possible interaction and correlation with glypican-3 hold the key.
|
| ジャーナル |
Placenta
|
| Abstract |
There have been controversies whether maternal serum placental protein 5 (PP5)/tissue factor pathway inhibitor (TFPI)-2 is increased in the patients with preeclampsia and/or intrauterine growth restriction (IUGR). Here, we have estimated the serum PP5/TFPI-2 in these patients by a sandwich enzyme-linked immunosorbent assay with a newly developed monoclonal antibody, coupled with placental immunohistochemical studies of their placentae with semiquantitative scoring. Serum PP5/TFPI-2 level was significantly elevated only in the patients with preeclampsia alone (p=0.033), while PP5/TFPI-2 was detected significantly less intensely in the placentae of the same patients (p=0.035) in immunohistochemistry, as compared to Controls. A proteoglycan present on the placental villous surface, glypican-3, showed the same pattern of staining as PP5/TFPI-2, and there was a positive correlation (C.I.=0.506, p=0.004) between the immunohistochemical scores for these. Further experiments using HepG2 cells transfected with PP5/TFPI-2 suggested that glypican-3 could anchor PP5/TFPI-2 on the placental villi. A possibility that a decrease in glypican-3 in the placenta increases the outflow of PP5/TFPI-2, which in turn increases its serum level, was proposed. Preeclampsia and IUGR, often regarded to have the same pathological basis in common, showed distinct distributions of PP5/TFPI-2, which could be a clue to elucidate the pathogenesis of preeclampsia and IUGR.
|
| 巻・号 |
28(2-3)
|
| ページ |
224-32
|
| 公開日 |
2007-1-1
|
| DOI |
10.1016/j.placenta.2006.01.023
|
| PII |
S0143-4004(06)00028-2
|
| PMID |
16580726
|
| MeSH |
Adult
Female
Fetal Growth Retardation / blood
Fetal Growth Retardation / metabolism*
Glycoproteins / blood
Glycoproteins / metabolism*
Glypicans / metabolism*
Humans
Immunohistochemistry
Infant, Newborn
Male
Placenta / metabolism*
Pre-Eclampsia / blood
Pre-Eclampsia / metabolism*
Pregnancy
Syndecan-1 / metabolism
|
| IF |
3.177
|
| 引用数 |
28
|
|
WOS 分野
|
OBSTETRICS & GYNECOLOGY
REPRODUCTIVE BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2 |
