RRC ID 42622
著者 Yokoyama S, Takeda K, Shibahara S.
タイトル SOX10, in combination with Sp1, regulates the endothelin receptor type B gene in human melanocyte lineage cells.
ジャーナル FEBS J
Abstract Waardenburg syndrome (WS) is an auditory-pigmentary disorder that exhibits varying combinations of sensorineural hearing loss and abnormal pigmentation of the hair and skin. WS type 4 (WS4), a subtype of WS, is characterized by the presence of the aganglionic megacolon and is associated with mutations in the gene encoding either endothelin 3, endothelin receptor type B (EDNRB), or Sry-box 10 (SOX10). Here, we provide evidence that SOX10 regulates the expression of EDNRB gene in human melanocyte-lineage cells, as judged by RNA interference and chromatin immunoprecipitation analyses. Human melanocytes preferentially express the EDNRB transcripts derived from the conventional EDNRB promoter. SOX10 transactivates the EDNRB promoter through the cis-acting elements, the two CA-rich sequences and the GC box. Moreover, a transcription factor Sp1 enhances the degree of the SOX10-mediated transactivation of the EDNRB promoter through these cis-acting elements. Furthermore, we have shown that the EDNRB promoter is heavily methylated in HeLa human cervical cancer cells, lacking EDNRB expression, but not in melanocytes and HMV-II melanoma cells. The expression of EDNRB became detectable in HeLa cells after treatment with a demethylating reagent, 5'-aza-2'-deoxycytidine, which was further enhanced in the transformed cells over-expressing SOX10. We therefore suggest that SOX10, alone or in combination with Sp1, regulates transcription of the EDNRB gene, thereby ensuring appropriate expression level of EDNRB in human melanocytes.
巻・号 273(8)
ページ 1805-20
公開日 2006-4-1
DOI 10.1111/j.1742-4658.2006.05200.x
PII EJB5200
PMID 16623715
MeSH Cell Lineage / genetics* Cells, Cultured Chromatin Immunoprecipitation DNA Primers / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation* HeLa Cells High Mobility Group Proteins / genetics High Mobility Group Proteins / metabolism* Humans Melanocytes / cytology Melanocytes / physiology* Promoter Regions, Genetic / genetics RNA, Small Interfering / pharmacology Receptor, Endothelin B / genetics* Receptor, Endothelin B / metabolism SOXE Transcription Factors Sp1 Transcription Factor / genetics Sp1 Transcription Factor / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* Transcriptional Activation
IF 4.392
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HMV-II(RCB0777)