RRC ID |
42629
|
著者 |
Hong J, Yokomakura A, Nakano Y, Ishihara K, Kaneda M, Onodera M, Nakahama K, Morita I, Niikura K, Ahn JW, Zee O, Ohuchi K.
|
タイトル |
Inhibition of vacuolar-type (H+)-ATPase by the cytostatic macrolide apicularen A and its role in apicularen A-induced apoptosis in RAW 264.7 cells.
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ジャーナル |
FEBS Lett
|
Abstract |
Apicularen A and the known vacuolar-type (H(+))-ATPase (V-ATPase) inhibitor bafilomycin A(1) induced apoptosis of RAW 264.7 cells, while apicularen B, an N-acetyl-glucosamine glycoside of apicularen A, was far less effective. Apicularen A inhibited vital staining with acridine orange of the intracellular organelles of RAW 264.7 cells, inhibited the ATP-dependent proton transport into inside-out microsome vesicles, and inhibited the bafilomycin A(1)-sensitive ATP hydrolysis. The IC(50) values of the proton transport were 0.58 nM for apicularen A, 13 nM for apicularen B, and 0.95 nM for bafilomycin A(1). Furthermore, apicularen A inhibited the bafilomycin A(1)-sensitive ATP hydrolysis more potently than apicularen B. F-ATPase and P-ATPase were not inhibited by apicularen A. We concluded that apicularen A inhibits V-ATPase, and thus induces apoptosis in RAW 264.7 cells.
|
巻・号 |
580(11)
|
ページ |
2723-30
|
公開日 |
2006-5-15
|
DOI |
10.1016/j.febslet.2006.04.031
|
PII |
S0014-5793(06)00466-2
|
PMID |
16647709
|
MeSH |
Adenosine Triphosphate / metabolism
Adenosine Triphosphate / pharmacology
Animals
Apoptosis / drug effects*
Bridged Bicyclo Compounds, Heterocyclic / chemistry
Bridged Bicyclo Compounds, Heterocyclic / pharmacology*
Cell Line
Cell Nucleus / drug effects
Cell Shape / drug effects
Enzyme Inhibitors / chemistry
Enzyme Inhibitors / pharmacology*
Macrolides / chemistry
Macrolides / pharmacology
Membrane Potentials / drug effects
Mice
Mitochondrial Membranes / drug effects
Proton-Translocating ATPases / antagonists & inhibitors*
Proton-Translocating ATPases / metabolism
|
IF |
3.057
|
引用数 |
16
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
A431 |