| RRC ID |
42732
|
| 著者 |
Wu Y, Kawate H, Ohnaka K, Nawata H, Takayanagi R.
|
| タイトル |
Nuclear compartmentalization of N-CoR and its interactions with steroid receptors.
|
| ジャーナル |
Mol Cell Biol
|
| Abstract |
The repression mechanisms by the nuclear receptor corepressor (N-CoR) of steroid hormone receptor (SHR)-mediated transactivation were examined. Yellow fluorescent protein (YFP)-N-CoR was distributed as intranuclear discrete dots, while coexpression of androgen receptor (AR), glucocorticoid receptor alpha, and estrogen receptor alpha ligand-dependently triggered redistribution of YFP-N-CoR. In fluorescence recovery after photobleaching analysis, mobility of the N-CoR was reduced by 5alpha-dihydrotestosterone (DHT)-bound AR. The middle region of N-CoR mostly contributed to the interaction with agonist-bound SHRs and the suppression of their transactivation function. N-CoR impaired the DHT-induced N-C interaction of AR, and the impaired interaction was dose-dependently recovered by coexpression of SRC-1 and CBP. N-CoR also impaired the intranuclear complete (distinct) focus formation of SHRs. Coexpression of SRC-1 or CBP released YFP-N-CoR or endogenous N-CoR from incomplete foci and simultaneously recovered complete foci of AR-green fluorescent protein. These results indicate that the relative ratio of coactivators and corepressors determines the conformational equilibrium between transcriptionally active and inactive SHRs in the presence of agonists. The intranuclear foci formed by agonist-bound SHRs were completely destroyed by actinomycin D and alpha-amanitin, indicating that the focus formation does not precede the transcriptional activation. The focus formation may reflect the accumulation of SHR/coactivator complexes released from the transcriptionally active sites and thus be a mirror of transcriptionally active complex formation.
|
| 巻・号 |
26(17)
|
| ページ |
6633-55
|
| 公開日 |
2006-9-1
|
| DOI |
10.1128/MCB.01534-05
|
| PII |
26/17/6633
|
| PMID |
16914745
|
| PMC |
PMC1592818
|
| MeSH |
3T3-L1 Cells
Animals
COS Cells
CREB-Binding Protein / metabolism
Cell Compartmentation*
Cell Line, Tumor
Cell Nucleus Structures / drug effects
Cell Nucleus Structures / metabolism*
Cells, Cultured
Chlorocebus aethiops
Dihydrotestosterone / pharmacology
Green Fluorescent Proteins / metabolism
Histone Acetyltransferases
Humans
Ligands
Mice
Mutant Proteins / metabolism
NIH 3T3 Cells
Nuclear Proteins / metabolism*
Nuclear Receptor Co-Repressor 1
Nuclear Receptor Coactivator 1
Protein Binding / drug effects
Protein Transport / drug effects
RNA Splicing / drug effects
Receptors, Androgen / metabolism
Receptors, Steroid / agonists
Receptors, Steroid / metabolism*
Repressor Proteins / metabolism*
Transcription Factors / metabolism
Transcriptional Activation / drug effects
|
| IF |
3.611
|
| 引用数 |
29
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
