Reference - Detail
|Author||Chen L, Fink T, Ebbesen P, Zachar V.|
|Title||Hypoxic treatment inhibits insulin-induced chondrogenesis of ATDC5 cells despite upregulation of DEC1.|
|Journal||Connect Tissue Res|
Chondrogenesis occurs in vivo in a hypoxic environment, in which the hypoxia inducible factor 1, HIF-1, plays a regulatory role, possibly mediated through the transcription factor DEC1. We have analyzed the effect of hypoxia (1% oxygen) alone and in combination with insulin on the chondrogenic differentiation of the mouse embryonic stem cell line ATDC5. Hypoxic treatment alone induced early chondrogenesis as evidenced by enhanced expression of aggrecan and collagen II, whereas hypoxic incubation of insulin-treated cells delayed and suppressed insulin-mediated early chondrogenesis and almost completely blocked hypertrophic differentiation. Paradoxically, the transcriptional activation of DEC1 was invariably enhanced by the hypoxic exposure.
|MeSH||Aggrecans Animals Basic Helix-Loop-Helix Transcription Factors / metabolism* Cartilage / drug effects* Cartilage / metabolism Cell Differentiation / drug effects Cell Line, Tumor Chondrocytes / drug effects* Chondrocytes / metabolism Chondrogenesis / drug effects* Chondrogenesis / physiology Chondroitin Sulfate Proteoglycans / genetics Chondroitin Sulfate Proteoglycans / metabolism Collagen Type II / genetics Collagen Type II / metabolism Collagen Type X / genetics Collagen Type X / metabolism Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism Homeodomain Proteins / metabolism* Hypoxia / metabolism* Insulin / pharmacology* Lectins, C-Type / genetics Lectins, C-Type / metabolism Mice Up-Regulation|
|WOS Category||ORTHOPEDICS CELL BIOLOGY|
|Human and Animal Cells|