RRC ID |
42735
|
著者 |
Ohgusu T, Hamase K, Tanaka H, Shoyama Y, Zaitsu K.
|
タイトル |
High-throughput determination of free D-aspartic acid in mammals by enzyme immunoassay using specific monoclonal antibody.
|
ジャーナル |
Anal Biochem
|
Abstract |
A method for rapid determination of free D-aspartic acid (D-Asp) in mammals has been established using a highly specific mouse monoclonal antibody against D-Asp for the first time. An anti-D-Asp monoclonal antibody was obtained by the immunization of bovine-serum-albumin-conjugated D-Asp to BALB/c mice. The obtained antibody has a high specificity toward D-Asp but shows a slight cross-reactivity to all other D- and L- amino acids including L-Asp. The calibration range of the competitive enzyme linked immunosorbent assay (ELISA) is 0.016-16 micromol/mL D-Asp in rat serum samples. The precisions of this method were evaluated by inter-plate and intraplate assays, and the relative standard deviation values were 4.8% and 4.5%, respectively. The values of D-Asp determined by the present ELISA have a good correlation to those determined by high-performance liquid chromatography with the correlation coefficient of 0.963. Using this ELISA, the time course of D-Asp in the rat serum after intravenous administration was successfully demonstrated. The present method provides a simple and high-throughput determination of D-Asp in mammals, and is a useful tool for clarifying the physiological roles and diagnostic values of this D-amino acid.
|
巻・号 |
357(1)
|
ページ |
15-20
|
公開日 |
2006-10-1
|
DOI |
10.1016/j.ab.2006.06.039
|
PII |
S0003-2697(06)00490-8
|
PMID |
16920063
|
MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology*
Aspartic Acid / blood*
Aspartic Acid / chemistry
Calibration
Cattle
Enzyme-Linked Immunosorbent Assay / methods*
Female
Injections, Intravenous
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Ovalbumin / chemistry
Rats
Sensitivity and Specificity
Serum Albumin, Bovine / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
|
IF |
2.877
|
引用数 |
4
|
WOS 分野
|
CHEMISTRY, ANALYTICAL
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
SP2/0-Ag14(RCB0209) |