RRC ID 42752
著者 Imamura T, Bando N, Yamanishi R.
タイトル Beta-carotene modulates the immunological function of RAW264, a murine macrophage cell line, by enhancing the level of intracellular glutathione.
ジャーナル Biosci Biotechnol Biochem
Abstract The activities of beta-carotene on redox status and the immune functions of RAW264 cells, a murine macrophage cell line, were investigated. Supplementation with beta-carotene for RAW264 cells resulted in apparently inconsistent redox indices: lipid peroxidation was enhanced but intracellular oxidation was moderately attenuated. Attenuated intracellular oxidation was endorsed by an increase in glutathione accompanied by up-regulated transcription of a subunit of gamma-glutamylcysteine synthetase, the rate-limiting enzyme for glutathione synthesis. alpha-Tocopherol, which can quench lipid peroxidation by free radical, neither inhibited that by beta-carotene nor influenced the intracellular redox status. Lipopolysaccharide-stimulated transcriptions of IL-1beta and IL-12 p40 in RAW264 were inhibited by beta-carotene but not by alpha-tocopherol. These results indicate that beta-carotene, which can modulate the intracellular redox status of macrophages by enhancing the level of intracellular glutathione, is related to the immune functions of macrophages.
巻・号 70(9)
ページ 2112-20
公開日 2006-9-1
DOI 10.1271/bbb.60056
PII JST.JSTAGE/bbb/60056
PMID 16960387
MeSH Animals Antioxidants / pharmacology* Glutamate-Cysteine Ligase / chemistry Glutamate-Cysteine Ligase / genetics Glutathione / immunology* Glutathione / metabolism Glutathione Disulfide / immunology Glutathione Disulfide / metabolism Interleukin-12 Subunit p40 / biosynthesis Interleukin-12 Subunit p40 / genetics Interleukin-1beta / biosynthesis Interleukin-1beta / genetics Lipid Peroxides / immunology Lipid Peroxides / metabolism Lipopolysaccharides / pharmacology Macrophages / drug effects* Macrophages / enzymology Macrophages / immunology Macrophages / metabolism Mice Oxidation-Reduction / drug effects RNA, Messenger / biosynthesis RNA, Messenger / genetics Reactive Oxygen Species / immunology Reactive Oxygen Species / metabolism Reverse Transcriptase Polymerase Chain Reaction Thiobarbituric Acid Reactive Substances / metabolism alpha-Tocopherol / metabolism beta Carotene / pharmacology*
IF 1.516
引用数 17
WOS 分野 CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)