論文 - 詳細
RRC ID | 42752 |
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著者 | Imamura T, Bando N, Yamanishi R. |
タイトル | Beta-carotene modulates the immunological function of RAW264, a murine macrophage cell line, by enhancing the level of intracellular glutathione. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
The activities of beta-carotene on redox status and the immune functions of RAW264 cells, a murine macrophage cell line, were investigated. Supplementation with beta-carotene for RAW264 cells resulted in apparently inconsistent redox indices: lipid peroxidation was enhanced but intracellular oxidation was moderately attenuated. Attenuated intracellular oxidation was endorsed by an increase in glutathione accompanied by up-regulated transcription of a subunit of gamma-glutamylcysteine synthetase, the rate-limiting enzyme for glutathione synthesis. alpha-Tocopherol, which can quench lipid peroxidation by free radical, neither inhibited that by beta-carotene nor influenced the intracellular redox status. Lipopolysaccharide-stimulated transcriptions of IL-1beta and IL-12 p40 in RAW264 were inhibited by beta-carotene but not by alpha-tocopherol. These results indicate that beta-carotene, which can modulate the intracellular redox status of macrophages by enhancing the level of intracellular glutathione, is related to the immune functions of macrophages. |
巻・号 | 70(9) |
ページ | 2112-20 |
公開日 | 2006-9-1 |
DOI | 10.1271/bbb.60056 |
PII | JST.JSTAGE/bbb/60056 |
PMID | 16960387 |
MeSH | Animals Antioxidants / pharmacology* Glutamate-Cysteine Ligase / chemistry Glutamate-Cysteine Ligase / genetics Glutathione / immunology* Glutathione / metabolism Glutathione Disulfide / immunology Glutathione Disulfide / metabolism Interleukin-12 Subunit p40 / biosynthesis Interleukin-12 Subunit p40 / genetics Interleukin-1beta / biosynthesis Interleukin-1beta / genetics Lipid Peroxides / immunology Lipid Peroxides / metabolism Lipopolysaccharides / pharmacology Macrophages / drug effects* Macrophages / enzymology Macrophages / immunology Macrophages / metabolism Mice Oxidation-Reduction / drug effects RNA, Messenger / biosynthesis RNA, Messenger / genetics Reactive Oxygen Species / immunology Reactive Oxygen Species / metabolism Reverse Transcriptase Polymerase Chain Reaction Thiobarbituric Acid Reactive Substances / metabolism alpha-Tocopherol / metabolism beta Carotene / pharmacology* |
IF | 1.516 |
引用数 | 17 |
WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |