RRC ID 42757
Author Yabuta N, Onda H, Watanabe M, Yoshioka N, Nagamori I, Funatsu T, Toji S, Tamai K, Nojima H.
Title Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest.
Journal Nucleic Acids Res
Abstract We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found that there are two kinds of G0 phases caused by serum starvation, namely, the shallow G0 (or G0/G1) and the deep G0 phases. The shallow G0 is induced by only a few hours of serum starvation, while deep G0 is generated after 3 days of serum starvation. We propose that mammalian cells enter deep G0 through a G0 gate, which is only opened on the third day of serum starvation. TIGA1, one of the uncharacterized TIGA genes, encodes a homolog of cyanate permease of bacteria and localizes in mitochondria. This suggests that Tiga1 is involved in the inorganic ion transport and metabolism needed to maintain the deep G0 phase. Ectopic expression of TIGA1 inhibited not only tumor cell proliferation but also anchorage-independent growth of cancer cell lines. A microsatellite marker, ENDL-1, allowed us to detect loss of heterozygosity around the TIGA1 gene region (5q21-22). Further analysis of the TIGA genes we have identified here may help us to better understand the mechanisms that regulate the G0 phase.
Volume 34(17)
Pages 4878-92
Published 2006-1-1
DOI 10.1093/nar/gkl651
PII gkl651
PMID 16973895
PMC PMC1635288
MeSH Amino Acid Sequence Animals Blotting, Western Cell Line Cell Line, Tumor Cell Proliferation Contact Inhibition Culture Media, Serum-Free Genes, Tumor Suppressor Humans Kinetics Loss of Heterozygosity Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / physiology Membrane Transport Proteins / chemistry Membrane Transport Proteins / genetics Membrane Transport Proteins / physiology* Mitochondrial Membrane Transport Proteins Mitochondrial Proteins / chemistry Mitochondrial Proteins / genetics Mitochondrial Proteins / physiology* Molecular Sequence Data Neoplasms / genetics Protein Structure, Tertiary RNA, Messenger / biosynthesis* RNA, Messenger / metabolism Resting Phase, Cell Cycle / genetics* Sequence Homology, Amino Acid Tumor Stem Cell Assay Up-Regulation*
IF 11.502
Times Cited 17
Human and Animal Cells