| RRC ID |
42757
|
| 著者 |
Yabuta N, Onda H, Watanabe M, Yoshioka N, Nagamori I, Funatsu T, Toji S, Tamai K, Nojima H.
|
| タイトル |
Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found that there are two kinds of G0 phases caused by serum starvation, namely, the shallow G0 (or G0/G1) and the deep G0 phases. The shallow G0 is induced by only a few hours of serum starvation, while deep G0 is generated after 3 days of serum starvation. We propose that mammalian cells enter deep G0 through a G0 gate, which is only opened on the third day of serum starvation. TIGA1, one of the uncharacterized TIGA genes, encodes a homolog of cyanate permease of bacteria and localizes in mitochondria. This suggests that Tiga1 is involved in the inorganic ion transport and metabolism needed to maintain the deep G0 phase. Ectopic expression of TIGA1 inhibited not only tumor cell proliferation but also anchorage-independent growth of cancer cell lines. A microsatellite marker, ENDL-1, allowed us to detect loss of heterozygosity around the TIGA1 gene region (5q21-22). Further analysis of the TIGA genes we have identified here may help us to better understand the mechanisms that regulate the G0 phase.
|
| 巻・号 |
34(17)
|
| ページ |
4878-92
|
| 公開日 |
2006-1-1
|
| DOI |
10.1093/nar/gkl651
|
| PII |
gkl651
|
| PMID |
16973895
|
| PMC |
PMC1635288
|
| MeSH |
Amino Acid Sequence
Animals
Blotting, Western
Cell Line
Cell Line, Tumor
Cell Proliferation
Contact Inhibition
Culture Media, Serum-Free
Genes, Tumor Suppressor
Humans
Kinetics
Loss of Heterozygosity
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / physiology
Membrane Transport Proteins / chemistry
Membrane Transport Proteins / genetics
Membrane Transport Proteins / physiology*
Mitochondrial Membrane Transport Proteins
Mitochondrial Proteins / chemistry
Mitochondrial Proteins / genetics
Mitochondrial Proteins / physiology*
Molecular Sequence Data
Neoplasms / genetics
Protein Structure, Tertiary
RNA, Long Noncoding
RNA, Messenger / biosynthesis*
RNA, Messenger / metabolism
Resting Phase, Cell Cycle / genetics*
Sequence Homology, Amino Acid
Tumor Stem Cell Assay
Up-Regulation*
|
| IF |
11.502
|
| 引用数 |
17
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
