| RRC ID |
42771
|
| 著者 |
Lixia S, Yao K, Kaijun W, Deqiang L, Huajun H, Xiangwei G, Baohong W, Wei Z, Jianling L, Wei W.
|
| タイトル |
Effects of 1.8 GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells.
|
| ジャーナル |
Mutat Res
|
| Abstract |
To investigate the DNA damage, expression of heat shock protein 70 (Hsp70) and cell proliferation of human lens epithelial cells (hLEC) after exposure to the 1.8 GHz radiofrequency field (RF) of a global system for mobile communications (GSM). An Xc-1800 RF exposure system was used to employ a GSM signal at 1.8 GHz (217 Hz amplitude-modulated) with the output power in the specific absorption rate (SAR) of 1, 2 and 3 W/kg. After 2 h exposure to RF, the DNA damage of hLEC was accessed by comet assay at five different incubation times: 0, 30, 60, 120 and 240 min, respectively. Western blot and RT-PCR were used to determine the expression of Hsp70 in hLECs after RF exposure. The proliferation rate of cells was evaluated by bromodeoxyuridine incorporation on days 0, 1 and 4 after exposure. The results show that the difference of DNA-breaks between the exposed and sham-exposed (control) groups induced by 1 and 2 W/kg irradiation were not significant at any incubation time point (P > 0.05). The DNA damage caused by 3 W/kg irradiation was significantly increased at the times of 0 and 30 min after exposure (P < 0.05), a phenomenon that could not be seen at the time points of 60, 120 or 240 min (P > 0.05). Detectable mRNA as well as protein expression of Hsp70 was found in all groups. Exposure at SARs of 2 and 3 W/kg for 2 h exhibited significantly increased Hsp70 protein expression (P < 0.05), while no change in Hsp70 mRNA expression could be found in any of the groups (P > 0.05). No difference of the cell proliferation rate between the sham-exposed and exposed cells was found at any exposure dose tested (P > 0.05). The results indicate that exposure to non-thermal dosages of RF for wireless communications can induce no or repairable DNA damage and the increased Hsp70 protein expression in hLECs occurred without change in the cell proliferation rate. The non-thermal stress response of Hsp70 protein increase to RF exposure might be involved in protecting hLEC from DNA damage and maintaining the cellular capacity for proliferation.
|
| 巻・号 |
602(1-2)
|
| ページ |
135-42
|
| 公開日 |
2006-12-1
|
| DOI |
10.1016/j.mrfmmm.2006.08.010
|
| PII |
S0027-5107(06)00269-7
|
| PMID |
17011595
|
| MeSH |
Bromodeoxyuridine / metabolism
Cell Phone
Cell Proliferation / radiation effects
Cells, Cultured
Comet Assay
DNA Damage*
DNA Repair / radiation effects
Epithelial Cells / metabolism
Epithelial Cells / radiation effects
HSP70 Heat-Shock Proteins / genetics*
HSP70 Heat-Shock Proteins / metabolism
Humans
Lens, Crystalline / metabolism
Lens, Crystalline / radiation effects*
Radio Waves*
|
| IF |
2.463
|
| 引用数 |
78
|
| リソース情報 |
| ヒト・動物細胞 |
SRA 01/04(RCB1591) |
