RRC ID 42778
Author Sano M, Genkai N, Yajima N, Tsuchiya N, Homma J, Tanaka R, Miki T, Yamanaka R.
Title Expression level of ECT2 proto-oncogene correlates with prognosis in glioma patients.
Journal Oncol Rep
Abstract The ECT2 (epithelial cell transforming sequence 2) proto-oncogene encodes a guanine nucleotide exchange factor for Rho GTPases, and regulates cytokinesis. ECT2 plays a critical role in Rho activation during cytokinesis, and thus may play a role in the pathogenesis of glioma. In this study, we investigated relationships between ECT2 expression, tumor histology, and prognosis in glioma patients. ECT2 mRNA expression was examined using quantitative real-time PCR, while its protein expression was examined by immunohistochemistry of 54 glioma tissue samples. Expressions of ECT2 mRNA and protein were markedly increased in high-grade gliomas compared to low-grade gliomas. Patients in whom expression of ECT2 mRNA and protein in tumor tissues was the lowest survived longer than patients who had higher expression. In vitro, ECT2 siRNA inhibited glioma cell proliferation and invasion. These data suggest that increased expression of ECT2 contribute to promotion of tumor invasiveness and progression, implying that evaluation of ECT2 expression is a prognostic marker for glioma patients.
Volume 16(5)
Pages 1093-8
Published 2006-11-1
PMID 17016598
MeSH Adult Aged Brain Neoplasms / genetics Brain Neoplasms / metabolism* Brain Neoplasms / pathology Cell Growth Processes / drug effects Cell Line, Tumor Female Gene Expression Glioma / genetics Glioma / metabolism* Glioma / pathology Humans Immunohistochemistry Male Middle Aged Prognosis Proto-Oncogene Mas Proto-Oncogene Proteins / biosynthesis* Proto-Oncogene Proteins / genetics RNA, Messenger / biosynthesis RNA, Messenger / genetics RNA, Small Interfering / genetics Transfection
IF 3.417
Times Cited 55
Human and Animal Cells T98G(RCB1954) GI-1(RCB0763)