| RRC ID |
42830
|
| Author |
Delton-Vandenbroucke I, Bouvier J, Makino A, Besson N, Pageaux JF, Lagarde M, Kobayashi T.
|
| Title |
Anti-bis(monoacylglycero)phosphate antibody accumulates acetylated LDL-derived cholesterol in cultured macrophages.
|
| Journal |
J Lipid Res
|
| Abstract |
Bis(monoacylglycero)phosphate (BMP), also called lysobisphosphatidic acid, is a phospholipid highly enriched in the internal membranes of multivesicular late endosomes, in which it forms specialized lipid domains. It has been suggested that BMP-rich membranes regulate cholesterol transport. Here, we examine the effects of an anti-BMP antibody on cholesterol metabolism and transport in two macrophage cell lines, RAW 264.7 and THP-1, during loading with acetylated low density lipoprotein (AcLDL). Anti-BMP antibody was internalized and accumulated in both macrophage cell types. Cholesterol staining with filipin and mass measurements indicate that AcLDL-stimulated accumulation of free cholesterol (FC) was enhanced in macrophages that had accumulated the antibody. Unlike the hydrophobic amine U18666A (3-beta-[2-(diethylamino)ethoxy]androst-5-en-17-one), esterification of AcLDL-derived cholesterol by ACAT was not modified after anti-BMP treatment. AcLDL loading led to an increase of FC in the plasma membrane. This increase was further enhanced in anti-BMP-treated macrophages. However, cholesterol efflux to HDL was reduced in antibody-treated cells. These results suggest that the accumulation of anti-BMP antibody alters cholesterol homeostasis in AcLDL-loaded macrophages.
|
| Volume |
48(3)
|
| Pages |
543-52
|
| Published |
2007-3-1
|
| DOI |
10.1194/jlr.M600266-JLR200
|
| PII |
S0022-2275(20)43230-4
|
| PMID |
17146116
|
| MeSH |
Animals
Antibodies / immunology
Antibodies / pharmacology*
Cell Line, Tumor
Cell Membrane / drug effects
Cell Membrane / metabolism
Cells, Cultured
Cholesterol Esters / metabolism
Cholesterol, LDL / metabolism*
Esterification / drug effects
Filipin / metabolism
Humans
Lipid Metabolism / drug effects
Lipoproteins, LDL / metabolism*
Lysophospholipids / immunology*
Macrophages / cytology
Macrophages / drug effects*
Macrophages / metabolism
Mice
Microscopy, Fluorescence
Monoglycerides / immunology*
|
| IF |
4.483
|
| Times Cited |
22
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
