論文 - 詳細
| RRC ID | 42830 |
|---|---|
| 著者 | Delton-Vandenbroucke I, Bouvier J, Makino A, Besson N, Pageaux JF, Lagarde M, Kobayashi T. |
| タイトル | Anti-bis(monoacylglycero)phosphate antibody accumulates acetylated LDL-derived cholesterol in cultured macrophages. |
| ジャーナル | J Lipid Res |
| Abstract |
Bis(monoacylglycero)phosphate (BMP), also called lysobisphosphatidic acid, is a phospholipid highly enriched in the internal membranes of multivesicular late endosomes, in which it forms specialized lipid domains. It has been suggested that BMP-rich membranes regulate cholesterol transport. Here, we examine the effects of an anti-BMP antibody on cholesterol metabolism and transport in two macrophage cell lines, RAW 264.7 and THP-1, during loading with acetylated low density lipoprotein (AcLDL). Anti-BMP antibody was internalized and accumulated in both macrophage cell types. Cholesterol staining with filipin and mass measurements indicate that AcLDL-stimulated accumulation of free cholesterol (FC) was enhanced in macrophages that had accumulated the antibody. Unlike the hydrophobic amine U18666A (3-beta-[2-(diethylamino)ethoxy]androst-5-en-17-one), esterification of AcLDL-derived cholesterol by ACAT was not modified after anti-BMP treatment. AcLDL loading led to an increase of FC in the plasma membrane. This increase was further enhanced in anti-BMP-treated macrophages. However, cholesterol efflux to HDL was reduced in antibody-treated cells. These results suggest that the accumulation of anti-BMP antibody alters cholesterol homeostasis in AcLDL-loaded macrophages. |
| 巻・号 | 48(3) |
| ページ | 543-52 |
| 公開日 | 2007-3-1 |
| DOI | 10.1194/jlr.M600266-JLR200 |
| PII | S0022-2275(20)43230-4 |
| PMID | 17146116 |
| MeSH | Animals Antibodies / immunology Antibodies / pharmacology* Cell Line, Tumor Cell Membrane / drug effects Cell Membrane / metabolism Cells, Cultured Cholesterol Esters / metabolism Cholesterol, LDL / metabolism* Esterification / drug effects Filipin / metabolism Humans Lipid Metabolism / drug effects Lipoproteins, LDL / metabolism* Lysophospholipids / immunology* Macrophages / cytology Macrophages / drug effects* Macrophages / metabolism Mice Microscopy, Fluorescence Monoglycerides / immunology* |
| IF | 4.483 |
| 引用数 | 22 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |