論文 - 詳細
| RRC ID | 42855 |
|---|---|
| 著者 | Sato MK, Takahashi M, Yazawa M. |
| タイトル | Two regions of the tail are necessary for the isoform-specific functions of nonmuscle myosin IIB. |
| ジャーナル | Mol Biol Cell |
| Abstract |
To function in the cell, nonmuscle myosin II molecules assemble into filaments through their C-terminal tails. Because myosin II isoforms most likely assemble into homo-filaments in vivo, it seems that some self-recognition mechanisms of individual myosin II isoforms should exist. Exogenous expression of myosin IIB rod fragment is thus expected to prevent the function of myosin IIB specifically. We expected to reveal some self-recognition sites of myosin IIB from the phenotype by expressing appropriate myosin IIB rod fragments. We expressed the C-terminal 305-residue rod fragment of the myosin IIB heavy chain (BRF305) in MRC-5 SV1 TG1 cells. As a result, unstable morphology was observed like MHC-IIB(-/-) fibroblasts. This phenotype was not observed in cells expressing BRF305 mutants: 1) with a defect in assembling, 2) lacking N-terminal 57 residues (N-57), or 3) lacking C-terminal 63 residues (C-63). A myosin IIA rod fragment ARF296 corresponding to BRF305 was not effective. However, the chimeric ARF296, in which the N-57 and C-63 of BRF305 were substituted for the corresponding regions of ARF296, acquired the ability to induce unstable morphology. We propose that the N-57 and C-63 of BRF305 are involved in self-recognition when myosin IIB molecules assemble into homo-filament. |
| 巻・号 | 18(3) |
| ページ | 1009-17 |
| 公開日 | 2007-3-1 |
| DOI | 10.1091/mbc.e06-08-0706 |
| PII | E06-08-0706 |
| PMID | 17202408 |
| PMC | PMC1805092 |
| MeSH | Actins / metabolism Amino Acid Sequence Cell Shape Cytoskeleton / metabolism Fibroblasts / cytology Genes, Dominant Green Fluorescent Proteins / metabolism Humans Immunoprecipitation Molecular Sequence Data Myosin Subfragments / chemistry Nonmuscle Myosin Type IIB / chemistry* Nonmuscle Myosin Type IIB / deficiency Nonmuscle Myosin Type IIB / metabolism* Phenotype Protein Binding Protein Isoforms / chemistry Protein Isoforms / metabolism Sequence Deletion Structure-Activity Relationship |
| IF | 3.791 |
| 引用数 | 24 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MRC-5 SV1 TG1(RCB0207) MRC-5(RCB0211) |