| RRC ID |
42859
|
| 著者 |
Narukawa M, Suzuki N, Takayama T, Yamashita Y, Otsuka K, Ito K.
|
| タイトル |
Enamel matrix derivative stimulates osteogenesis- and chondrogenesis-related transcription factors in C3H10T1/2 cells.
|
| ジャーナル |
Acta Biochim Biophys Sin (Shanghai)
|
| Abstract |
Our purpose was to determine how enamel matrix derivative (EMD) affects the expression of osteogenesis- and chondrogenesis-related transcription factors in undifferentiated mesenchymal cells. C3H10T1/2 cell line, a typical pluripotential mesenchymal cell line, was cultured with or without EMD for up to 7 d. Expression of mRNAs encoding osteogenesis- and chondrogenesis-related transcription factors (Runx2, Osterix, AJ18, Dlx5, Msx2, Sox5, Sox9 and Zfp60) was measured using real-time polymerase chain reaction. Runx2 and Sox9 protein expression and the presence of bone morphogenetic protein (BMP)-6-like molecules in EMD were determined by Western blotting. EMD substantially increased mRNA levels of osteogenesis- and chondrogenesis-related transcription factors. EMD also induced Runx2 and Sox9 protein expression. Western blotting analysis of EMD using anti-BMP-6 antibody revealed immunoreactive bands corresponding to about 14 kDa and 60 kDa. These results suggest that EMD stimulates osteogenesis- and chondrogenesis-related transcription factors, and these activities may be mediated, at least in part, by BMP-6 in EMD.
|
| 巻・号 |
39(1)
|
| ページ |
1-7
|
| 公開日 |
2007-1-1
|
| DOI |
10.1111/j.1745-7270.2007.00250.x
|
| PMID |
17213952
|
| MeSH |
Animals
Bone Morphogenetic Protein 6
Bone Morphogenetic Proteins / analysis
Cells, Cultured
Chondrogenesis / genetics*
Collagen Type II / genetics
Dental Enamel Proteins / pharmacology*
Gene Expression Regulation / drug effects*
Mice
Osteocalcin / genetics
Osteogenesis / genetics*
RNA, Messenger / analysis
|
| IF |
2.836
|
| 引用数 |
13
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
