論文 - 詳細
| RRC ID | 42859 |
|---|---|
| 著者 | Narukawa M, Suzuki N, Takayama T, Yamashita Y, Otsuka K, Ito K. |
| タイトル | Enamel matrix derivative stimulates osteogenesis- and chondrogenesis-related transcription factors in C3H10T1/2 cells. |
| ジャーナル | Acta Biochim Biophys Sin (Shanghai) |
| Abstract |
Our purpose was to determine how enamel matrix derivative (EMD) affects the expression of osteogenesis- and chondrogenesis-related transcription factors in undifferentiated mesenchymal cells. C3H10T1/2 cell line, a typical pluripotential mesenchymal cell line, was cultured with or without EMD for up to 7 d. Expression of mRNAs encoding osteogenesis- and chondrogenesis-related transcription factors (Runx2, Osterix, AJ18, Dlx5, Msx2, Sox5, Sox9 and Zfp60) was measured using real-time polymerase chain reaction. Runx2 and Sox9 protein expression and the presence of bone morphogenetic protein (BMP)-6-like molecules in EMD were determined by Western blotting. EMD substantially increased mRNA levels of osteogenesis- and chondrogenesis-related transcription factors. EMD also induced Runx2 and Sox9 protein expression. Western blotting analysis of EMD using anti-BMP-6 antibody revealed immunoreactive bands corresponding to about 14 kDa and 60 kDa. These results suggest that EMD stimulates osteogenesis- and chondrogenesis-related transcription factors, and these activities may be mediated, at least in part, by BMP-6 in EMD. |
| 巻・号 | 39(1) |
| ページ | 1-7 |
| 公開日 | 2007-1-1 |
| DOI | 10.1111/j.1745-7270.2007.00250.x |
| PMID | 17213952 |
| MeSH | Animals Bone Morphogenetic Protein 6 Bone Morphogenetic Proteins / analysis Cells, Cultured Chondrogenesis / genetics* Collagen Type II / genetics Dental Enamel Proteins / pharmacology* Gene Expression Regulation / drug effects* Mice Osteocalcin / genetics Osteogenesis / genetics* RNA, Messenger / analysis |
| IF | 2.836 |
| 引用数 | 13 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |