論文 - 詳細
| RRC ID | 42867 |
|---|---|
| 著者 | Ohtsuka K, Ohnishi H, Fujiwara M, Kishino T, Matsushima S, Furuyashiki G, Takei H, Koshiishi Y, Goya T, Watanabe T. |
| タイトル | Abnormalities of epidermal growth factor receptor in lung squamous-cell carcinomas, adenosquamous carcinomas, and large-cell carcinomas: tyrosine kinase domain mutations are not rare in tumors with an adenocarcinoma component. |
| ジャーナル | Cancer |
| Abstract |
BACKGROUND:Tyrosine kinase domain (TKD) gene mutations of the epidermal growth factor receptor gene (EGFR) have proven to be clinically significant in nonsmall-cell lung cancer (NSCLC), particularly in adenocarcinoma. However, TKD mutations together with deletion mutations in the extracellular domain of EGFR (EGFRvIII) have not been fully investigated in NSCLC except for adenocarcinoma. The present study sought to gain further insight into the significance of EGFR mutations in NSCLC by focusing on nonadenocarcinoma NSCLC. METHODS:EGFR TKD mutations were investigated using direct sequencing and mutation-specific polymerase chain reaction (PCR), and EGFRvIII mutations were examined using reverse transcriptase-PCR in samples from 42 NSCLC patients and 6 NSCLC cell lines excluding adenocarcinoma. RESULTS:EGFR TKD mutations were detected in 1 of 7 (14%) squamous-cell carcinomas with an adenocarcinoma component and 2 of 4 (50%) adenosquamous carcinomas. In contrast, EGFR TKD mutations were not identified in 24 pure squamous-cell carcinomas without any adenocarcinoma component, 7 large-cell carcinomas, or 6 cell lines. EGFRvIII was detected solely in 1 of 7 large-cell carcinomas (14%), but not in 31 squamous-cell carcinomas, 4 adenosquamous carcinomas, or 6 cell lines. CONCLUSIONS:These results suggest that EGFR TKD mutations are found in NSCLCs with an adenocarcinoma element. Patients with such lesions are thus considered candidates for molecular therapies targeting EGFR. |
| 巻・号 | 109(4) |
| ページ | 741-50 |
| 公開日 | 2007-2-15 |
| DOI | 10.1002/cncr.22476 |
| PMID | 17238183 |
| MeSH | Adult Aged Aged, 80 and over Carcinoma, Adenosquamous / drug therapy Carcinoma, Adenosquamous / genetics* Carcinoma, Large Cell / drug therapy Carcinoma, Large Cell / genetics* Carcinoma, Non-Small-Cell Lung / drug therapy Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Squamous Cell / drug therapy Carcinoma, Squamous Cell / genetics* DNA Mutational Analysis ErbB Receptors / genetics* Female Humans Lung Neoplasms / drug therapy Lung Neoplasms / genetics Male Middle Aged Mutation* Neoplasm Staging Prognosis Protein Structure, Tertiary Protein-Tyrosine Kinases / chemistry Survival Rate |
| IF | 5.772 |
| 引用数 | 56 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |