論文 - 詳細
RRC ID | 42868 |
---|---|
著者 | Takano Y, Yamauchi K, Hayakawa K, Hiramatsu N, Kasai A, Okamura M, Yokouchi M, Shitamura A, Yao J, Kitamura M. |
タイトル | Transcriptional suppression of nephrin in podocytes by macrophages: roles of inflammatory cytokines and involvement of the PI3K/Akt pathway. |
ジャーナル | FEBS Lett |
Abstract |
Expression of nephrin, a crucial component of the glomerular slit diaphragm, is downregulated in patients with proteinuric glomerular diseases. Using conditionally immortalized reporter podocytes, we found that bystander macrophages as well as macrophage-derived cytokines IL-1beta and TNF-alpha markedly suppressed activity of the nephrin gene promoter in podocytes. The cytokine-initiated repression was reversible, observed on both basal and inducible expression, independent of Wilms' tumor suppressor WT1, and caused in part via activation of the phosphatidylinositol-3-kinase/Akt pathway. These results indicated a novel mechanism by which activated macrophages participate in the induction of proteinuria in glomerular diseases. |
巻・号 | 581(3) |
ページ | 421-6 |
公開日 | 2007-2-6 |
DOI | 10.1016/j.febslet.2006.12.051 |
PII | S0014-5793(07)00020-8 |
PMID | 17239861 |
MeSH | Animals Cell Communication Cells, Cultured Cytokines / metabolism Cytokines / pharmacology Down-Regulation / drug effects Humans Inflammation Mediators / metabolism Inflammation Mediators / pharmacology Interleukin-1beta / metabolism Interleukin-1beta / pharmacology Macrophages / immunology Macrophages / metabolism* Membrane Proteins / genetics* Mice Phosphatidylinositol 3-Kinases / metabolism Podocytes / drug effects Podocytes / metabolism* Promoter Regions, Genetic Proto-Oncogene Proteins c-akt / metabolism Tumor Necrosis Factor-alpha / metabolism Tumor Necrosis Factor-alpha / pharmacology |
IF | 3.057 |
引用数 | 65 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | J774.1(RCB0434) |