RRC ID 42868
著者 Takano Y, Yamauchi K, Hayakawa K, Hiramatsu N, Kasai A, Okamura M, Yokouchi M, Shitamura A, Yao J, Kitamura M.
タイトル Transcriptional suppression of nephrin in podocytes by macrophages: roles of inflammatory cytokines and involvement of the PI3K/Akt pathway.
ジャーナル FEBS Lett
Abstract Expression of nephrin, a crucial component of the glomerular slit diaphragm, is downregulated in patients with proteinuric glomerular diseases. Using conditionally immortalized reporter podocytes, we found that bystander macrophages as well as macrophage-derived cytokines IL-1beta and TNF-alpha markedly suppressed activity of the nephrin gene promoter in podocytes. The cytokine-initiated repression was reversible, observed on both basal and inducible expression, independent of Wilms' tumor suppressor WT1, and caused in part via activation of the phosphatidylinositol-3-kinase/Akt pathway. These results indicated a novel mechanism by which activated macrophages participate in the induction of proteinuria in glomerular diseases.
巻・号 581(3)
ページ 421-6
公開日 2007-2-6
DOI 10.1016/j.febslet.2006.12.051
PII S0014-5793(07)00020-8
PMID 17239861
MeSH Animals Cell Communication Cells, Cultured Cytokines / metabolism Cytokines / pharmacology Down-Regulation / drug effects Humans Inflammation Mediators / metabolism Inflammation Mediators / pharmacology Interleukin-1beta / metabolism Interleukin-1beta / pharmacology Macrophages / immunology Macrophages / metabolism* Membrane Proteins / genetics* Mice Phosphatidylinositol 3-Kinases / metabolism Podocytes / drug effects Podocytes / metabolism* Promoter Regions, Genetic Proto-Oncogene Proteins c-akt / metabolism Tumor Necrosis Factor-alpha / metabolism Tumor Necrosis Factor-alpha / pharmacology
IF 3.057
引用数 65
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 J774.1(RCB0434)