RRC ID 42869
著者 Tsuruta T, Oh-Hashi K, Ueno Y, Kitade Y, Kiuchi K, Hirata Y.
タイトル RNAi knockdown of caspase-activated DNase inhibits rotenone-induced DNA fragmentation in HeLa cells.
ジャーナル Neurochem Int
Abstract Rotenone, an inhibitor of mitochondrial complex I, induces apoptosis in a variety of cells. However, little is known about endogenous endonucleases involved in rotenone-induced DNA fragmentation, a biochemical hallmark of apoptosis. We used a chemically modified siRNA which is thought to be more effective than a non-modified siRNA to study whether caspase-activated DNase (CAD) contributes to this phenomenon. Western blot analysis showed that CAD protein decreased to 8% of control levels in human cervical carcinoma HeLa cells after a 48h transfection of siRNA. Consistent with the reduction of the protein level, the siRNA was found to inhibit rotenone-induced DNA fragmentation. These results suggest that CAD is the endogenous endonuclease that mediates internucleosomal DNA degradation in rotenone-induced apoptosis.
巻・号 50(4)
ページ 601-6
公開日 2007-3-1
DOI 10.1016/j.neuint.2006.12.002
PII S0197-0186(06)00354-8
PMID 17239993
MeSH Apoptosis / drug effects Apoptosis / genetics* Caspases / metabolism* DNA / drug effects DNA / genetics DNA / metabolism DNA Fragmentation / drug effects* Deoxyribonucleases / genetics* Deoxyribonucleases / metabolism* Down-Regulation / physiology Gene Expression Regulation, Enzymologic / genetics HeLa Cells Humans Nucleosomes / genetics Nucleosomes / metabolism RNA Interference / physiology RNA, Small Interfering / genetics* Rotenone / pharmacology Transfection / methods Uncoupling Agents / pharmacology
IF 3.881
引用数 6
WOS 分野 NEUROSCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞