| RRC ID |
42869
|
| Author |
Tsuruta T, Oh-Hashi K, Ueno Y, Kitade Y, Kiuchi K, Hirata Y.
|
| Title |
RNAi knockdown of caspase-activated DNase inhibits rotenone-induced DNA fragmentation in HeLa cells.
|
| Journal |
Neurochem Int
|
| Abstract |
Rotenone, an inhibitor of mitochondrial complex I, induces apoptosis in a variety of cells. However, little is known about endogenous endonucleases involved in rotenone-induced DNA fragmentation, a biochemical hallmark of apoptosis. We used a chemically modified siRNA which is thought to be more effective than a non-modified siRNA to study whether caspase-activated DNase (CAD) contributes to this phenomenon. Western blot analysis showed that CAD protein decreased to 8% of control levels in human cervical carcinoma HeLa cells after a 48h transfection of siRNA. Consistent with the reduction of the protein level, the siRNA was found to inhibit rotenone-induced DNA fragmentation. These results suggest that CAD is the endogenous endonuclease that mediates internucleosomal DNA degradation in rotenone-induced apoptosis.
|
| Volume |
50(4)
|
| Pages |
601-6
|
| Published |
2007-3-1
|
| DOI |
10.1016/j.neuint.2006.12.002
|
| PII |
S0197-0186(06)00354-8
|
| PMID |
17239993
|
| MeSH |
Apoptosis / drug effects
Apoptosis / genetics*
Caspases / metabolism*
DNA / drug effects
DNA / genetics
DNA / metabolism
DNA Fragmentation / drug effects*
Deoxyribonucleases / genetics*
Deoxyribonucleases / metabolism*
Down-Regulation / physiology
Gene Expression Regulation, Enzymologic / genetics
HeLa Cells
Humans
Nucleosomes / genetics
Nucleosomes / metabolism
RNA Interference / physiology
RNA, Small Interfering / genetics*
Rotenone / pharmacology
Transfection / methods
Uncoupling Agents / pharmacology
|
| IF |
3.881
|
| Times Cited |
6
|
|
WOS Category
|
NEUROSCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
