Reference - Detail
|Author||Ishihara K, Takahashi A, Kaneko M, Sugeno H, Hirasawa N, Hong J, Zee O, Ohuchi K.|
|Title||Differentiation of eosinophilic leukemia EoL-1 cells into eosinophils induced by histone deacetylase inhibitors.|
EoL-1 cells differentiate into eosinophils in the presence of n-butyrate, but the mechanism has remained to be elucidated. Because n-butyrate can inhibit histone deacetylases, we hypothesized that the inhibition of histone deacetylases induces the differentiation of EoL-1 cells into eosinophils. In this study, using n-butyrate and two other histone deacetylase inhibitors, apicidin and trichostatin A, we have analyzed the relationship between the inhibition of histone deacetylases and the differentiation into eosinophils in EoL-1 cells. It was demonstrated that apicidin and n-butyrate induced a continuous acetylation of histones H4 and H3, inhibited the proliferation of EoL-1 cells without attenuating the level of FIP1L1-PDGFRA mRNA, and induced the expression of markers for mature eosinophils such as integrin beta7, CCR1, and CCR3 on EoL-1 cells, while trichostatin A evoked a transient acetylation of histones and induced no differentiation into eosinophils. These findings suggest that the continuous inhibition of histone deacetylases in EoL-1 cells induces the differentiation into mature eosinophils.
|MeSH||Acetylation / drug effects Butyrates / pharmacology Cell Differentiation / drug effects* Cell Proliferation / drug effects Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology* Eosinophils / drug effects* Eosinophils / enzymology Gene Expression Regulation, Enzymologic / drug effects HL-60 Cells Histone Deacetylase Inhibitors* Histone Deacetylases / metabolism Histones / metabolism Humans Hydroxamic Acids / pharmacology Hypereosinophilic Syndrome / drug therapy* Hypereosinophilic Syndrome / enzymology Peptides, Cyclic / pharmacology Platelet-Derived Growth Factor / genetics Platelet-Derived Growth Factor / metabolism RNA, Messenger / metabolism mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism|
|WOS Category||MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY|
|Human and Animal Cells|