論文 - 詳細
| RRC ID | 42878 |
|---|---|
| 著者 | Ishihara K, Takahashi A, Kaneko M, Sugeno H, Hirasawa N, Hong J, Zee O, Ohuchi K. |
| タイトル | Differentiation of eosinophilic leukemia EoL-1 cells into eosinophils induced by histone deacetylase inhibitors. |
| ジャーナル | Life Sci |
| Abstract |
EoL-1 cells differentiate into eosinophils in the presence of n-butyrate, but the mechanism has remained to be elucidated. Because n-butyrate can inhibit histone deacetylases, we hypothesized that the inhibition of histone deacetylases induces the differentiation of EoL-1 cells into eosinophils. In this study, using n-butyrate and two other histone deacetylase inhibitors, apicidin and trichostatin A, we have analyzed the relationship between the inhibition of histone deacetylases and the differentiation into eosinophils in EoL-1 cells. It was demonstrated that apicidin and n-butyrate induced a continuous acetylation of histones H4 and H3, inhibited the proliferation of EoL-1 cells without attenuating the level of FIP1L1-PDGFRA mRNA, and induced the expression of markers for mature eosinophils such as integrin beta7, CCR1, and CCR3 on EoL-1 cells, while trichostatin A evoked a transient acetylation of histones and induced no differentiation into eosinophils. These findings suggest that the continuous inhibition of histone deacetylases in EoL-1 cells induces the differentiation into mature eosinophils. |
| 巻・号 | 80(13) |
| ページ | 1213-20 |
| 公開日 | 2007-3-6 |
| DOI | 10.1016/j.lfs.2006.12.016 |
| PII | S0024-3205(07)00014-8 |
| PMID | 17258775 |
| MeSH | Acetylation / drug effects Butyrates / pharmacology Cell Differentiation / drug effects* Cell Proliferation / drug effects Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology* Eosinophils / drug effects* Eosinophils / enzymology Gene Expression Regulation, Enzymologic / drug effects HL-60 Cells Histone Deacetylase Inhibitors* Histone Deacetylases / metabolism Histones / metabolism Humans Hydroxamic Acids / pharmacology Hypereosinophilic Syndrome / drug therapy* Hypereosinophilic Syndrome / enzymology Peptides, Cyclic / pharmacology Platelet-Derived Growth Factor / genetics Platelet-Derived Growth Factor / metabolism RNA, Messenger / metabolism mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism |
| IF | 3.647 |
| 引用数 | 19 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | |