RRC ID 42926
Author Amano S, Arai M, Goto S, Togari A.
Title Inhibitory effect of NPY on isoprenaline-induced osteoclastogenesis in mouse bone marrow cells.
Journal Biochim Biophys Acta
Abstract The effect of neuropeptide Y (NPY), a co-transmitter with noradrenaline in peripheral sympathetic nerve fibers, on the osteoclastogenesis in mouse bone marrow cell cultures treated with isoprenaline, a beta-adrenergic receptor (beta-AR) agonist, was examined. The mouse bone marrow cells constitutively expressed mRNAs for the NPY-Y1 receptor and beta2-AR. NPY inhibited the formation of osteoclast-like cells induced by isoprenaline but not that by 1alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)2D3) or soluble receptor activator of nuclear factor-kappaB ligand (RANKL); and it suppressed the production of RANKL and cyclic AMP (cAMP) increased by isoprenaline but not those increased by 1alpha,25(OH)2D3. NPY also inhibited osteoclastogenesis induced by forskolin, an activator of adenylate cyclase; however, it did not inhibit that induced by exogenously supplied dibutyryl cAMP, a cell-permeable cAMP analog that activates cAMP-dependent protein kinase. These results demonstrate that NPY inhibited the isoprenaline-induced osteoclastogenesis by blocking the agonist-elicited increases in the production of cAMP and RANKL in mouse bone marrow cells, suggesting an interaction between NPY and beta-AR agonist in bone resorption.
Volume 1770(6)
Pages 966-73
Published 2007-6-1
DOI 10.1016/j.bbagen.2007.02.009
PII S0304-4165(07)00058-X
PMID 17383824
MeSH Adenylyl Cyclase Inhibitors Adenylyl Cyclases / metabolism Adrenergic beta-Agonists / pharmacology* Animals Bone Marrow Cells / cytology Bone Marrow Cells / metabolism* Bone Resorption / metabolism* Bucladesine / metabolism Bucladesine / pharmacology Calcitriol / metabolism Calcitriol / pharmacology Cells, Cultured Colforsin / pharmacology Enzyme Activation / drug effects Isoproterenol / pharmacology* Male Mice Mice, Mutant Strains Neuropeptide Y / metabolism Neuropeptide Y / pharmacology* Osteoclasts / cytology Osteoclasts / metabolism* RANK Ligand / metabolism Receptors, Adrenergic, beta-2 Receptors, Neuropeptide Y / agonists Receptors, Neuropeptide Y / metabolism Vitamins / metabolism Vitamins / pharmacology
IF 3.411
Times Cited 34
Human and Animal Cells ST2(RCB0224)