Reference - Detail
| RRC ID | 4295 |
|---|---|
| Author | Shim J, Lee J. |
| Title | Regulation of rnt-1 expression mediated by the opposing effects of BRO-1 and DBL-1 in the nematode Caenorhabditis elegans. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
During development of Caenorhabditis elegans, expression of the RUNX homolog, rnt-1, is tightly regulated both spatially and temporally. In this study, we investigated the mechanism underlying the temporal regulation of rnt-1. We found that rnt-1 contained evolutionarily conserved consensus RUNX binding sequences within one of its introns, and that RNT-1 bound to these intronic sequences both in vitro and in vivo in the presence of BRO-1, suggesting that RNT-1 together with BRO-1 represses its own transcription. Fine deletion and substitution experiments revealed a binding site within the intron that was critical for rnt-1 regulation. Importantly, we found that the TGFbeta homolog, DBL-1, was required for counteracting the repressive activity of BRO-1 at postembryonic stages. Accordingly, ectopic expression of DBL-1 induced transcription of rnt-1 in the lateral hypodermis and other tissues even at the postembryonic stages. Taken together, our data suggest that rnt-1 expression is regulated by the balance between DBL-1-mediated activation and BRO-1-mediated repression at the postembryonic stages. |
| Volume | 367(1) |
| Pages | 130-6 |
| Published | 2008-2-29 |
| DOI | 10.1016/j.bbrc.2007.12.097 |
| PII | S0006-291X(07)02751-9 |
| PMID | 18158917 |
| MeSH | Animals Binding Sites Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology* Neuropeptides / genetics Neuropeptides / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism* Subcutaneous Tissue / metabolism Transcription Factors / genetics Transcription Factors / metabolism* Transforming Growth Factor beta / genetics Transforming Growth Factor beta / metabolism* |
| IF | 2.985 |
| Times Cited | 3 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| C.elegans | tm491 tm388 |