RRC ID 4296
Author Takata N, Itoh B, Misaki K, Hirose T, Yonemura S, Okada M.
Title Non-receptor tyrosine kinase CSK-1 controls pharyngeal muscle organization in Caenorhabditis elegans.
Journal Genes Cells
Abstract C-terminal Src kinase (Csk) is a non-receptor type of tyrosine kinase, and serves as an essential negative regulator of Src family tyrosine kinases (SFKs) in vertebrates. However, analyses of Csk and SFKs from primitive animals suggest that the Csk-mediated mechanisms regulating SFK activity might diverge between evolutional branches, different tissues or SFK family members. We examined in vivo roles of CSK-1, a Caenorhabditis elegans orthologue of Csk, by generating animals lacking csk-1 function. Although some csk-1 mutants died during embryogenesis, the majority of mutants died during the first stage of larval development. In csk-1 mutants, the function of pharyngeal muscles, the major site of CSK-1 expression, was severely damaged. The pumping of pharyngeal grinder cells became arrhythmic, causing disabled feeding. Electron microscopy showed that pharyngeal muscle filaments were disorientated in the csk-1 mutants. These indicate that CSK-1 is crucial for proper organization of pharyngeal muscles. However, the growth arrest phenotype in csk-1 mutants could not be suppressed by src-1 and/or src-2 mutation, and SRC-1 was not significantly activated in the csk-1 mutants. These results suggest that CSK-1 has an essential function in organization of pharyngeal muscle filaments that does not require C. elegans SFKs.
Volume 14(3)
Pages 381-93
Published 2009-3
DOI 10.1111/j.1365-2443.2008.01275.x
PII GTC1275
PMID 19210548
MeSH Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Embryo, Nonmammalian / metabolism Gene Deletion Pharyngeal Muscles / embryology Pharyngeal Muscles / metabolism Protein Kinases / genetics Protein Kinases / metabolism*
IF 2.048
Times Cited 2
WOS Category GENETICS & HEREDITY CELL BIOLOGY
Resource
C.elegans tm1916