論文 - 詳細
| RRC ID | 42989 |
|---|---|
| 著者 | Zhao S, Zhang J, Zhang X, Dong X, Sun X. |
| タイトル | Arsenic trioxide induces different gene expression profiles of genes related to growth and apoptosis in glioma cells dependent on the p53 status. |
| ジャーナル | Mol Biol Rep |
| Abstract |
We have previously reported that As(2)O(3) affected cell cycle progression and cyclins D1 and B1 expression in two glioma cell lines differing in p53 status (U87MG-wt; T98G-mutated). In the present study, we further demonstrated that As(2)O(3) affected proliferation, viability and apoptosis of the two cell lines in a dose- and time-dependent manner, and T98G cells were more sensitive than U87MG cells to As(2)O(3) -induced apoptosis and inhibition of proliferation and viability. We further investigated the expression profiles of genes related with apoptosis and cell cycle in the two cell lines with a human cDNA-microarray (SuperArray) spotted with 267 genes of apoptosis and cell cycle. Thirty five genes were upregulated and 15 genes downregulated at least 2-fold by As(2)O(3) in U87-MG cells; whereas, 38 genes were upregulated and 21 genes downregulated at least 2-fold in T98G cells by As(2)O(3). After As(2)O(3) treatment, p53 expression was upregulated 56.5-fold in T98G cells, but only 6.0-fold in U87MG cells. The results indicate that As(2)O(3) suppresses the growth of U87MG cells mainly by regulating expression of genes of cell cycle arrest, stress and toxicity; whereas As(2)O(3) affects T98G cells mainly by regulating expression of genes belonging to Bcl-2, tumor necrotic factor receptor and ligand families. The data may be helpful for optimizing As(2)O(3) as an anti-cancer drug in the treatment of gliomas. |
| 巻・号 | 35(3) |
| ページ | 421-9 |
| 公開日 | 2008-9-1 |
| DOI | 10.1007/s11033-007-9102-6 |
| PMID | 17530438 |
| MeSH | Apoptosis / drug effects* Arsenic Trioxide Arsenicals / pharmacology* Cell Line, Tumor Gene Expression Profiling Gene Expression Regulation, Neoplastic / drug effects* Glioma / genetics* Glioma / pathology* Humans Oxides / pharmacology* RNA, Messenger / genetics Tumor Suppressor Protein p53 / genetics* |
| IF | 1.402 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U-87 MG(RCB0419) T98G(RCB1954) |