| RRC ID |
42996
|
| Author |
Tsuboi K, Moritake T, Tsuchida Y, Tokuuye K, Matsumura A, Ando K.
|
| Title |
Cell cycle checkpoint and apoptosis induction in glioblastoma cells and fibroblasts irradiated with carbon beam.
|
| Journal |
J Radiat Res
|
| Abstract |
This study was conducted in order to evaluate the cytotoxicity of high linear-energy-transfer (LET) ionizing radiation (IR) on glioblastoma cells and fibroblasts using different modes of cell inactivation assays. Two human glioblastoma cell lines with or without p53-mutation, and fibroblasts were used as materials. Gamma rays and 290 MeV/u carbon beams with LET values of 20, 40, 80 keV/mum were used. To evaluate cell inactivation, we used colony formation assay, morphological detection of apoptosis, and flow-cytometry. Serial expressions of p53 and p21 were analyzed by immunoblotting. High-LET IR reduced the reproductive potency of these cells to identical levels in spite of differences in gamma-sensitivity, and yield of cell death correlated to LET values. A p53-wild-type glioblastoma cell line demonstrated a higher yield of apoptosis than other cell lines, whereas fibroblasts hardly displayed any cell death indicating senescence-like growth arrest even after high LET IR. A p53-mutant tumor cell line demonstrated very low yield of cell death with prominent G2/M arrest. Results of radiosensitivity differ according to what mode of cell inactivation is selected. While fibroblasts depend on G1 block after IR, G2/M blocks may play crucial roles in the radioresistance of p53-mutant glioblastoma cells.
|
| Volume |
48(4)
|
| Pages |
317-25
|
| Published |
2007-7-1
|
| DOI |
10.1269/jrr.06081
|
| PII |
JST.JSTAGE/jrr/06081
|
| PMID |
17548940
|
| MeSH |
Apoptosis*
Carbon / chemistry*
Cell Cycle / radiation effects*
Cell Line, Tumor
Dose-Response Relationship, Drug
Fibroblasts / metabolism
Fibroblasts / radiation effects*
Flow Cytometry
Gene Expression Regulation, Neoplastic / radiation effects*
Genes, p53
Glioblastoma / radiotherapy*
Humans
Linear Energy Transfer
Microscopy, Fluorescence
Mutation
Radiation, Ionizing
|
| IF |
1.95
|
| Times Cited |
40
|
|
WOS Category
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
