Reference - Detail
| RRC ID | 43065 |
|---|---|
| Author | Ikeda Y, Adachi Y, Ishii T, Tamura H, Aketagawa J, Tanaka S, Ohno N. |
| Title | Blocking effect of anti-Dectin-1 antibodies on the anti-tumor activity of 1,3-beta-glucan and the binding of Dectin-1 to 1,3-beta-glucan. |
| Journal | Biol Pharm Bull |
| Abstract |
Schizophyllan (SPG) is used to treat cervical cancer in combination with irradiation to enhance the immunological surveillance system. Dectin-1 is a cell surface receptor for 1,3-beta-glucan. In this study, we prepared two anti-Dectin-1 monoclonal antibodies, 4B2 and SC30 having a K(D) of 7.04 x 10(-8) M and 1.55 x 10(-7) M, respectively, and evaluated the role of Dectin-1 in SPG-induced anti-tumor activity in mice. Expression of Dectin-1 on peritoneal macrophages and binding of SPG to the cells were decreased by administration of 4B2 and SC30. SPG-mediated anti-tumor activity was inhibited by 4B2 and SC30. 4B2 and SC30 inhibited the binding of SPG to splenocytes from mice. The binding of SPG-biotin to Dectin-1-transfected HEK293 cells was inhibited by 4B2, but not SC30. 4B2 and SC30 differ in their influence on Dectin-1 between primary cells and transduced cells, and Dectin-1 effects 1,3-beta-glucan-mediated anti-tumor activity in mice by binding to SPG. |
| Volume | 30(8) |
| Pages | 1384-9 |
| Published | 2007-8-1 |
| DOI | 10.1248/bpb.30.1384 |
| PII | JST.JSTAGE/bpb/30.1384 |
| PMID | 17666790 |
| MeSH | Animals Antibodies, Blocking / pharmacology* Antibodies, Monoclonal / pharmacology* Antineoplastic Agents / pharmacology* Antineoplastic Agents, Phytogenic / chemistry Antineoplastic Agents, Phytogenic / pharmacology Biotin / chemistry Biotin / pharmacology Cells, Cultured Flow Cytometry Hybridomas / metabolism Lectins, C-Type Male Membrane Proteins / antagonists & inhibitors* Membrane Proteins / metabolism* Mice Mice, Inbred ICR Nerve Tissue Proteins / antagonists & inhibitors* Nerve Tissue Proteins / metabolism* Peritoneal Cavity / cytology Sizofiran / chemistry Sizofiran / pharmacology Spleen / cytology Transfection beta-Glucans / antagonists & inhibitors* beta-Glucans / metabolism beta-Glucans / pharmacology* |
| IF | 1.863 |
| Times Cited | 18 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |