論文 - 詳細
| RRC ID | 43176 |
|---|---|
| 著者 | Kumarevel T, Sakamoto K, Gopinath SC, Shinkai A, Kumar PK, Yokoyama S. |
| タイトル | Crystal structure of an archaeal specific DNA-binding protein (Ape10b2) from Aeropyrum pernix K1. |
| ジャーナル | Proteins |
| Abstract |
DNA binding proteins are essential in all organisms, and they play important roles in both compacting and regulating the genetic material. All thermophilic and hyperthermophilic archaea encode one or more copies of Alba or Sso10b, which is a small, abundant, basic protein that binds DNA. Here, we present the crystal structure of Ape10b2 from Aeropyrum pernix K1 at 1.70 A. Although the overall structure resembles the known Alba protein fold, a significant conformational change was observed in the loop regions. Specifically, the L5 loop is slightly longer, as compared to those of other known proteins, and the flexibility of this loop may facilitate the interaction with double stranded DNA. In addition, we showed that Ape10b2 binds to 16 and 39 bp duplex DNAs with high affinity. On the basis of our analyses, we have created a putative protein-DNA complex model. |
| 巻・号 | 71(3) |
| ページ | 1156-62 |
| 公開日 | 2008-5-15 |
| DOI | 10.1002/prot.21807 |
| PMID | 18004791 |
| MeSH | Aeropyrum* / chemistry Aeropyrum* / metabolism Amino Acid Sequence Archaeal Proteins / chemistry* Archaeal Proteins / metabolism Crystallography, X-Ray / methods DNA, Archaeal / chemistry* DNA, Archaeal / metabolism DNA-Binding Proteins / chemistry* DNA-Binding Proteins / metabolism Models, Molecular Molecular Sequence Data Protein Conformation Protein Folding |
| IF | 2.828 |
| 引用数 | 17 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |