| RRC ID |
43196
|
| 著者 |
Kobayashi K, Nagahama M, Ohkubo N, Kojima T, Shirai H, Iwamoto S, Oda M, Sakurai J.
|
| タイトル |
Role of Ca2+-binding motif in cytotoxicity induced by Clostridium perfringens iota-toxin.
|
| ジャーナル |
Microb Pathog
|
| Abstract |
Clostridium perfringens iota-toxin is a binary toxin composed of an enzymatic component (Ia) and a binding component (Ib). We investigated the role of the conserved Ca(2+)-binding motif of Ib in the cytotoxicity of iota-toxin. The cytotoxicity of iota-toxin increased with an increase in the concentration of extracellular Ca(2+). A surface plasmon resonance analysis showed that the binding of Ia to the oligomer of Ib is dependent on the concentration of Ca(2+). However, the addition of Ca(2+) had no effect on the binding of (125)I-labeled Ib to the cells. We replaced Asp-8, -10, and -12 in the Ca(2+)-binding motif of Ib with alanine. D8A, D10A, and D12A bound to the cell and formed an oligomer at about half of the wild-type Ib. The cytotoxicity of Ib variants in the presence of Ia was about 500-fold less than that of wild-type Ib. Immunofluorescence study showed that these variants were internalized in the early endosomes like wild-type Ib. However, wild-type Ib-induced internalization of Ia in the cells, but these variants did not. The result indicates that the conserved Ca(2+)-binding motif in the N-terminal region of Ib plays a role in the interaction of Ib with Ia in the presence of Ca(2+).
|
| 巻・号 |
44(4)
|
| ページ |
265-70
|
| 公開日 |
2008-4-1
|
| DOI |
10.1016/j.micpath.2007.10.010
|
| PII |
S0882-4010(07)00143-X
|
| PMID |
18060735
|
| MeSH |
ADP Ribose Transferases / chemistry
ADP Ribose Transferases / genetics
ADP Ribose Transferases / metabolism*
ADP Ribose Transferases / toxicity*
Amino Acid Motifs
Amino Acid Sequence
Amino Acid Substitution
Animals
Bacterial Toxins / chemistry
Bacterial Toxins / genetics
Bacterial Toxins / metabolism*
Bacterial Toxins / toxicity*
Binding Sites
Calcium / metabolism*
Chlorocebus aethiops
Clostridium perfringens / metabolism*
Conserved Sequence
Dogs
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Binding
Sequence Alignment
Surface Plasmon Resonance
Vero Cells
|
| IF |
2.914
|
| 引用数 |
11
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Vero(RCB0001)
MDCK(RCB0995) |
