| RRC ID |
43200
|
| Author |
Hirata I, Nomura Y, Ito M, Shimazu A, Okazaki M.
|
| Title |
Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds.
|
| Journal |
J Artif Organs
|
| Abstract |
The development is expected of scaffold biomaterials that feature a shape-maintaining property in addition to high porosity and large pores that cells can easily invade. To develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized carbonate apatite (CO3Ap) was mixed with neutralized collagen gel, and the CO3Ap-collagen mixtures were lyophilized into sponges in a porous hydroxyapatite (HAp) frame ring. X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR) analyses together with chemical analysis indicated that the synthesized CO3Ap had a crystalline nature and a chemical composition similar to that of bone. Scanning electron microscope (SEM) observation showed that the CO3Ap-collagen sponge had a sui pore size for cell invasion. In proliferation and differentiation experiments with osteoblasts, alkaline phosphatase and osteopontin activity were clearly detected. When these sponge-frame complexes with bone morphogenic protein (rh-BMP2) were implanted beneath the periosteum cranii of rats, significant new bone was created at the surface of the periosteum cranii after 4 weeks of implantation. These reinforced CO3Ap-collagen sponges with rh-BMP2 are expected to be used as hard tissue scaffold biomaterials for the therapeutic purpose of the rapid cure of bone defects.
|
| Volume |
10(4)
|
| Pages |
212-7
|
| Published |
2007-1-1
|
| DOI |
10.1007/s10047-007-0391-2
|
| PMID |
18071850
|
| MeSH |
Absorbable Implants*
Animals
Apatites / chemistry*
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / pharmacology*
Carbonates / chemistry*
Cattle
Cell Culture Techniques
Collagen / chemistry*
Gels
Humans
Male
Mice
Microscopy, Electron, Scanning
Osteogenesis*
Porosity
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Spectroscopy, Fourier Transform Infrared
Tissue Engineering*
Transforming Growth Factor beta / pharmacology*
X-Ray Diffraction
|
| IF |
1.223
|
| Times Cited |
17
|
|
WOS Category
|
TRANSPLANTATION
ENGINEERING, BIOMEDICAL
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
