| RRC ID |
43203
|
| Author |
Kanemura N, Tsurumi H, Okuno M, Matsushima-Nishiwaki R, Shimizu M, Moriwaki H.
|
| Title |
Retinoid X receptor alpha is highly phosphorylated in retinoic acid-resistant HL-60R cells and the combination of 9-cis retinoic acid plus MEK inhibitor induces apoptosis in the cells.
|
| Journal |
Leuk Res
|
| Abstract |
We examined the effects of 9-cis retinoic acid (RA) on the expression levels of retinoid X receptor alpha (RXR alpha) and its phosphorylated form (p-RXR alpha) in HL-60 and HL-60R cells. 9-cis RA reduced both RXR alpha and p-RXR alpha in HL-60 cells, but did neither in HL-60R cells. However, when the HL-60R cells were treated with the combination of 9-cis RA plus PD98059, MEK inhibitor, the p-RXR alpha and RXR alpha proteins all markedly decreased. Moreover, the combination of those agents induced apoptosis in HL-60R cells. Phosphorylation of RXR alpha might be associated with RA-resistance in HL-60R cells.
|
| Volume |
32(6)
|
| Pages |
884-92
|
| Published |
2008-6-1
|
| DOI |
10.1016/j.leukres.2007.11.005
|
| PII |
S0145-2126(07)00438-9
|
| PMID |
18082883
|
| MeSH |
Alitretinoin
Apoptosis / drug effects*
Apoptosis / physiology
Blotting, Western
Butadienes / pharmacology
Cell Proliferation / drug effects
Drug Resistance, Neoplasm*
Drug Therapy, Combination
Enzyme Inhibitors / pharmacology
Flavonoids / pharmacology
HL-60 Cells
Humans
Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors*
Mitogen-Activated Protein Kinase Kinases / metabolism
Nitriles / pharmacology
Phosphorylation / drug effects
Retinoid X Receptor alpha / metabolism*
Tretinoin / pharmacology*
|
| IF |
2.214
|
| Times Cited |
13
|
|
WOS Category
|
HEMATOLOGY
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
HL60(RCB0041) |
