RRC ID 43213
著者 Li HW, Yao K, Jin HY, Sun LX, Lu DQ, Yu YB.
タイトル Proteomic analysis of human lens epithelial cells exposed to microwaves.
ジャーナル Jpn J Ophthalmol
Abstract PURPOSE:To study proteomic changes in human lens epithelial cells (HLECs) exposed to 1800-MHz Global System for Mobile Communication (GSM)-like microwaves.
METHODS:In three separate experiments, HLECs were exposed and sham-exposed (six dishes each) to 1800-MHz GSM-like radiation for 2 h. The specific absorption rates were 1.0, 2.0, or 3.5 W/kg. Immediately after radiation, the proteome was extracted from the HLECs. Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis(2-DE; silver staining) and PDQuest 2-DE analysis software were used to separate and analyze the proteome of exposed and sham-exposed HLECs. Four differentially expressed protein spots were selected and identified by using electrospray ionization tandem mass spectrometry (ESI-MS-MS).
RESULTS:When the protein profiles of exposed cells were compared with those of sham-exposed cells, four proteins were detected as upregulated. After analysis by ESI-MS-MS and through a database search, heat-shock protein (HSP) 70 and heterogeneous nuclear ribonucleoprotein K (hnRNP K) were determined to be upregulated in the exposed cells.
CONCLUSIONS:Two-dimensional polyacrylamide gel electrophoresis combined with mass spectrometry may be a powerful tool for screening potential electromagnetic-reaction protein markers. HSP70 and hnRNP K are involved in the stress reaction of HLECs exposed to microwaves. These cell responses are nonthermal effects of the electromagnetic field.
巻・号 51(6)
ページ 412-6
公開日 2007-1-1
DOI 10.1007/s10384-007-0483-9
PMID 18158590
MeSH Cells, Cultured Crystallins / metabolism* Electrophoresis, Gel, Two-Dimensional Epithelial Cells / metabolism Epithelial Cells / radiation effects HSP70 Heat-Shock Proteins / metabolism Heterogeneous-Nuclear Ribonucleoprotein K Humans Hydrogen-Ion Concentration Lens, Crystalline / metabolism* Lens, Crystalline / radiation effects* Microwaves* Proteome / metabolism* Proteomics Ribonucleoproteins / metabolism Spectrometry, Mass, Electrospray Ionization Up-Regulation
IF 1.725
引用数 22
WOS 分野 OPHTHALMOLOGY
リソース情報
ヒト・動物細胞