| RRC ID |
43226
|
| Author |
Boros S, Wilmarth PA, Kamps B, de Jong WW, Bloemendal H, Lampi K, Boelens WC.
|
| Title |
Tissue transglutaminase catalyzes the deamidation of glutamines in lens betaB(2)- and betaB(3)-crystallins.
|
| Journal |
Exp Eye Res
|
| Abstract |
Tissue transglutaminase (tTG) is a Ca(2+)-dependent enzyme catalyzing the formation of covalent crosslinks between peptide-bound glutamine and lysine residues. Lens crystallins, including alphaB-crystallin and several beta-crystallins, are in vitro substrates for tTG. In both human and bovine fetal lens extracts treated with commercially available guinea pig liver tTG we detected the formation of high molecular weight (HMW) aggregates containing crosslinked betaB(2)- and betaA(3)-crystallin. More interestingly, 2D-gel electrophoresis combined with mass spectrometry analysis revealed that glutamines present in the N-terminal arms of betaB(2)- and betaB(3)-crystallins deamidate readily in the presence of tTG. We found that both tTG-catalyzed crosslinking and deamidation disrupt the beta-crystallin complex, suggesting that these tTG-catalyzed modifications can influence the macromolecular assembly of lens crystallins. These data together suggest that tTG can contribute to the age-related deamidation of glutamine residues of lens crystallins.
|
| Volume |
86(2)
|
| Pages |
383-93
|
| Published |
2008-2-1
|
| DOI |
10.1016/j.exer.2007.11.011
|
| PII |
S0014-4835(07)00340-5
|
| PMID |
18184610
|
| MeSH |
Aging / metabolism
Amides / metabolism
Animals
Catalysis
Cattle
Fetus / metabolism
GTP-Binding Proteins / pharmacology*
Glutamine / metabolism*
Humans
In Vitro Techniques
Lens, Crystalline / drug effects*
Lens, Crystalline / embryology
Lens, Crystalline / metabolism
Middle Aged
Protein Glutamine gamma Glutamyltransferase 2
Proteome / drug effects
Transglutaminases / pharmacology*
beta-Crystallin B Chain / metabolism*
|
| IF |
3.011
|
| Times Cited |
16
|
|
WOS Category
|
OPHTHALMOLOGY
|
| Resource |
| Human and Animal Cells |
anti-αB-crystallin(RCB1304) |
