論文 - 詳細
| RRC ID | 43295 |
|---|---|
| 著者 | Takano M, Satoh C, Kunimatsu N, Otani M, Hamada-Kanazawa M, Miyake M, Ming K, Yayama K, Okamoto H. |
| タイトル | Lipopolysaccharide activates the kallikrein-kinin system in mouse choroid plexus cell line ECPC4. |
| ジャーナル | Neurosci Lett |
| Abstract |
Regulation of the kallikrein-kinin system in cerebral inflammation is still unclear. Here, we used reverse-transcription polymerase chain reaction (RT-PCR) techniques to show that lipopolysaccharide (LPS) activates the kallikrein-kinin system by enhancing liberation of bradykinin (BK), and alters mRNA levels of kallikrein-kinin system components, including high molecular weight (H-) and low molecular weight (L-) kininogens, in ECPC4 cells, a cell line of mouse choroid plexus epithelium. LPS treatment increased liberation of immunoreactive bradykinin in the supernatant of ECPC4 cells, and addition of LPS (500 ng/ml) to cultures resulted in elevation of H- and L-kininogen mRNA levels in ECPC4 cells within 24-48 h. Furthermore, LPS treatment elevated bradykinin type 2 and type 1 receptor mRNA levels within 4h, but did not change tissue kallikrein or plasma kallikrein mRNA levels. On the other hand, expression of pro-inflammatory mediators interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and cyclooxygenase-2 mRNA increased within 4-8h after addition of LPS to ECPC4 cells. The addition of IL-1beta and TNF-alpha to investigate the major mediator for kininogen expression in ECPC4 cells remarkably induced expression of H- and L-kininogen mRNAs in ECPC4 cells. These results suggest that LPS activates the kallikrein-kinin system in the choroid plexus via autocrine induction of IL-1beta and TNF-alpha. |
| 巻・号 | 434(3) |
| ページ | 310-4 |
| 公開日 | 2008-4-4 |
| DOI | 10.1016/j.neulet.2008.01.072 |
| PII | S0304-3940(08)00156-0 |
| PMID | 18329172 |
| MeSH | Animals Bradykinin / cerebrospinal fluid Bradykinin / drug effects Bradykinin / genetics Cell Line Cerebrospinal Fluid / metabolism Choroid Plexus / drug effects Choroid Plexus / metabolism* Choroid Plexus / physiopathology Cyclooxygenase 2 / genetics Cytokines / genetics* Encephalitis / cerebrospinal fluid* Encephalitis / chemically induced Encephalitis / physiopathology Inflammation Mediators / metabolism* Interleukin-1beta / genetics Interleukin-1beta / pharmacology Kallikreins / cerebrospinal fluid* Kallikreins / drug effects Kallikreins / genetics Kinins / cerebrospinal fluid* Kinins / drug effects Kinins / genetics Lipopolysaccharides Mice RNA, Messenger / drug effects RNA, Messenger / metabolism Receptors, Bradykinin / drug effects Receptors, Bradykinin / metabolism Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / pharmacology Up-Regulation / drug effects Up-Regulation / physiology |
| IF | 2.274 |
| 引用数 | 9 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |