論文 - 詳細
| RRC ID | 43327 |
|---|---|
| 著者 | Ma J, Sawai H, Matsuo Y, Ochi N, Yasuda A, Takahashi H, Wakasugi T, Funahashi H, Sato M, Okada Y, Takeyama H, Manabe T. |
| タイトル | Interleukin-1alpha enhances angiogenesis and is associated with liver metastatic potential in human gastric cancer cell lines. |
| ジャーナル | J Surg Res |
| Abstract |
BACKGROUND:To better understand the underlying mechanism of liver metastasis formation in human gastric cancer, we evaluated the angiogenic capabilities of human gastric cancer cell lines with different metastatic potentials as well as the role of interleukin (IL)-1alpha in the angiogenic process. MATERIALS AND METHODS:Reverse transcription-polymerase chain reaction was used to detect the expression of IL-1alpha and vascular endothelial growth factor (VEGF) mRNA in gastric cancer cell lines with different liver metastatic potentials. Levels of VEGF secreted by human gastric cancer cells were measured by enzyme-linked immunosorbent assay. We also examined how gastric cancer cells with different metastatic potentials influence the proliferation and tube formation of human umbilical vein endothelial cells (HUVECs) using the Premix WST-1 cell proliferation assay system and an angiogenesis assay, respectively. RESULTS:IL-1alpha expression levels were significantly correlated with liver metastatic potential in gastric cancer cell lines. Levels of VEGF secreted by gastric cancer cells appear to be regulated by IL-1alpha through IL-1 receptor Type 1 and were correlated with liver metastatic potential. Both HUVEC proliferation and tube formation were strongly enhanced by coculture with high liver-metastatic gastric cancer cells and were enhanced to a similar extent by culture in the presence of IL-1alpha. In contrast, blockade of IL-1alpha inhibited both HUVEC proliferation and angiogenesis. CONCLUSIONS:IL-1alpha may play a role in liver metastasis of gastric cancer via enhanced vascular endothelial cell proliferation and angiogenesis. |
| 巻・号 | 148(2) |
| ページ | 197-204 |
| 公開日 | 2008-8-1 |
| DOI | 10.1016/j.jss.2007.08.014 |
| PII | S0022-4804(07)00511-2 |
| PMID | 18395750 |
| MeSH | Cell Line, Tumor Cell Proliferation / drug effects Coculture Techniques Endothelium, Vascular / cytology Endothelium, Vascular / metabolism Humans Interleukin-1alpha / physiology* Liver Neoplasms / secondary* Neovascularization, Pathologic / physiopathology* RNA, Messenger / metabolism Receptors, Interleukin-1 / metabolism Stomach Neoplasms / blood supply* Stomach Neoplasms / pathology Up-Regulation / drug effects Vascular Endothelial Growth Factor A / metabolism |
| IF | 1.841 |
| 引用数 | 31 |
| WOS 分野 | SURGERY |
| リソース情報 | |
| ヒト・動物細胞 | |