RRC ID 43337
著者 Kouro T, Yokota T, Welner R, Kincade PW.
タイトル In vitro differentiation and measurement of B cell progenitor activity in culture.
ジャーナル Curr Protoc Immunol
Abstract As well as other blood cells, B lymphocytes originate from hematopoietic stem cells. However, it is not fully understood how their production is controlled. In the serum-free, stromal-cell-free B cell differentiation culture described here, early steps of the B lineage differentiation process are reproduced under defined conditions. This assay is useful for examining the direct effects of various soluble factors on B cell progenitors because it does not contain stromal cells or unknown factors. Additionally, this assay yields sufficient cloning to measure B cell progenitors from single cell cultures. Stromal cell coculture assays are also described that cover a wider category of precursors such as human B cell progenitors.
巻・号 Chapter 22
ページ Unit 22F.2
公開日 2005-5-1
DOI 10.1002/0471142735.im22f02s66
PMID 18432949
MeSH Animals B-Lymphocytes / cytology* Cell Differentiation Cells, Cultured Clinical Laboratory Techniques* Mice Stem Cells / cytology*
リソース情報
ヒト・動物細胞