RRC ID |
43337
|
著者 |
Kouro T, Yokota T, Welner R, Kincade PW.
|
タイトル |
In vitro differentiation and measurement of B cell progenitor activity in culture.
|
ジャーナル |
Curr Protoc Immunol
|
Abstract |
As well as other blood cells, B lymphocytes originate from hematopoietic stem cells. However, it is not fully understood how their production is controlled. In the serum-free, stromal-cell-free B cell differentiation culture described here, early steps of the B lineage differentiation process are reproduced under defined conditions. This assay is useful for examining the direct effects of various soluble factors on B cell progenitors because it does not contain stromal cells or unknown factors. Additionally, this assay yields sufficient cloning to measure B cell progenitors from single cell cultures. Stromal cell coculture assays are also described that cover a wider category of precursors such as human B cell progenitors.
|
巻・号 |
Chapter 22
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ページ |
Unit 22F.2
|
公開日 |
2005-5-1
|
DOI |
10.1002/0471142735.im22f02s66
|
PMID |
18432949
|
MeSH |
Animals
B-Lymphocytes / cytology*
Cell Differentiation
Cells, Cultured
Clinical Laboratory Techniques*
Mice
Stem Cells / cytology*
|
リソース情報 |
ヒト・動物細胞 |
|