論文 - 詳細
| RRC ID | 43337 |
|---|---|
| 著者 | Kouro T, Yokota T, Welner R, Kincade PW. |
| タイトル | In vitro differentiation and measurement of B cell progenitor activity in culture. |
| ジャーナル | Curr Protoc Immunol |
| Abstract |
As well as other blood cells, B lymphocytes originate from hematopoietic stem cells. However, it is not fully understood how their production is controlled. In the serum-free, stromal-cell-free B cell differentiation culture described here, early steps of the B lineage differentiation process are reproduced under defined conditions. This assay is useful for examining the direct effects of various soluble factors on B cell progenitors because it does not contain stromal cells or unknown factors. Additionally, this assay yields sufficient cloning to measure B cell progenitors from single cell cultures. Stromal cell coculture assays are also described that cover a wider category of precursors such as human B cell progenitors. |
| 巻・号 | Chapter 22 |
| ページ | Unit 22F.2 |
| 公開日 | 2005-5-1 |
| DOI | 10.1002/0471142735.im22f02s66 |
| PMID | 18432949 |
| MeSH | Animals B-Lymphocytes / cytology* Cell Differentiation Cells, Cultured Clinical Laboratory Techniques* Mice Stem Cells / cytology* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |