| RRC ID |
43371
|
| 著者 |
Wu S, Nishiyama N, Kano MR, Morishita Y, Miyazono K, Itaka K, Chung UI, Kataoka K.
|
| タイトル |
Enhancement of angiogenesis through stabilization of hypoxia-inducible factor-1 by silencing prolyl hydroxylase domain-2 gene.
|
| ジャーナル |
Mol Ther
|
| Abstract |
Hypoxia-inducible factor-1 (HIF-1) plays a central role in cellular response to hypoxia by activating vascular endothelial growth factor (VEGF) and other angiogenic factors. Prolyl hydroxylase domain-2 (PHD2) protein induces the degradation of HIF-1 by hydroxylating specific prolyl residues. Therefore gene silencing of PHD2 by RNA interference (RNAi) might increase the expression of angiogenic growth factors and, consequently, neoangiogenesis through the stabilization of HIF-1alpha. In this study we have shown that the specific silencing of PHD2 is sufficient for stabilizing HIF-1alpha and increasing its transcriptional activity, resulting in the increased expression of angiogenic factors including VEGF and fibroblast growth factor-2 (FGF2). Moreover, when PHD2-siRNA vector was used, the increase in VEGF secretion was observed for as long as 18 days after transfection. In vitro treatment of human umbilical vein endothelical cells with conditioned medium from PHD2-siRNA vector-transfected NIH3T3 cells was shown to increase cell proliferation. Also, in vivo angiogenesis was observed in mice implanted with Matrigel plugs mixed with NIH3T3 cells transfected with PHD2-siRNA vector. These results indicate that PHD2 silencing induces expressions of multiple angiogenic growth factors by stabilizing HIF-1alpha, and that the implantation of cells transfected with PHD2-siRNA vector is sufficient to enhance angiogenesis in vivo. In the light of these findings, PHD2 silencing by RNAi might offer a potential tool for angiogenic therapy.
|
| 巻・号 |
16(7)
|
| ページ |
1227-34
|
| 公開日 |
2008-7-1
|
| DOI |
10.1038/mt.2008.90
|
| PII |
S1525-0016(16)32462-5
|
| PMID |
18500250
|
| MeSH |
Animals
Cell Proliferation
DNA-Binding Proteins / antagonists & inhibitors*
DNA-Binding Proteins / genetics
Female
Gene Expression Regulation
Genetic Therapy*
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / genetics
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
Hypoxia-Inducible Factor-Proline Dioxygenases
Immediate-Early Proteins / antagonists & inhibitors*
Immediate-Early Proteins / genetics
Mice
Mice, Inbred BALB C
NIH 3T3 Cells
Neovascularization, Physiologic* / genetics
Procollagen-Proline Dioxygenase
RNA Interference
RNA, Small Interfering / genetics
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
8.986
|
| 引用数 |
33
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| リソース情報 |
| ヒト・動物細胞 |
NIH3T3 |
