Reference - Detail
| RRC ID | 43423 |
|---|---|
| Author | Nakamura K, Miyazato A, Koguchi Y, Adachi Y, Ohno N, Saijo S, Iwakura Y, Takeda K, Akira S, Fujita J, Ishii K, Kaku M, Kawakami K. |
| Title | Toll-like receptor 2 (TLR2) and dectin-1 contribute to the production of IL-12p40 by bone marrow-derived dendritic cells infected with Penicillium marneffei. |
| Journal | Microbes Infect |
| Abstract |
The present study was designed to elucidate the role of TLR2, TLR4 and dectin-1 in the production of IL-12p40 by bone marrow-derived dendritic cells (BM-DCs) infected with Penicillium marneffei. IL-12p40 production was almost completely abrogated in BM-DCs from TLR2 gene-knockout (KO) and MyD88KO mice, but not from TLR4-defective C3H/HeJ mice compared to those from control mice. Furthermore, BM-DCs from dectin-1KO mice faintly produced IL-12p40 upon stimulation with this fungus. Using a luciferase reporter assay, P. marneffei activated NF-kappaB in HEK293 cells transfected with the TLR2 gene, but not with the dectin-1 gene, and their co-transfection did not lead to further increase in this response. These results indicate that TLR2 and dectin-1 are essential in sensing P. marneffei for the activation of BM-DCs. |
| Volume | 10(10-11) |
| Pages | 1223-7 |
| Published | 2008-1-1 |
| DOI | 10.1016/j.micinf.2008.06.011 |
| PII | S1286-4579(08)00173-1 |
| PMID | 18652908 |
| MeSH | Analysis of Variance Animals Bone Marrow Cells / immunology Bone Marrow Cells / metabolism Bone Marrow Cells / microbiology Dendritic Cells / immunology* Dendritic Cells / metabolism Dendritic Cells / microbiology* Interleukin-12 Subunit p40 / biosynthesis* Lectins, C-Type Luciferases Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Inbred C57BL Mice, Knockout NF-kappa B / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Penicillium / immunology* Penicillium / metabolism Penicillium / pathogenicity Toll-Like Receptor 2 / genetics Toll-Like Receptor 2 / metabolism* Toll-Like Receptor 4 / genetics Toll-Like Receptor 4 / metabolism |
| IF | 2.373 |
| Times Cited | 14 |
| WOS Category | INFECTIOUS DISEASES IMMUNOLOGY MICROBIOLOGY |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |