| RRC ID |
43426
|
| Author |
Noman AS, Koide N, Khuda II, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
|
| Title |
Thalidomide inhibits epidermal growth factor-induced cell growth in mouse and human monocytic leukemia cells via Ras inactivation.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The effect of thalidomide on epidermal growth factor (EGF)-induced cell growth was examined. Thalidomide inhibited EGF-induced cell growth in mouse and human monocytic leukemia cells, RAW 264.7, U937 and THP-1. Thalidomide inhibited EGF-induced phosphorylation of extracellular signal regulated kinase (ERK) 1/2, but not p38 and stress-activated protein kinase (SAPK)/JNK. The phosphorylation of MEK1/2 and Raf at Ser 338 as the upstream molecules of ERK 1/2 was also prevented by thalidomide. Further, it inhibited EGF-induced Ras activation through preventing the transition to GTP-bound active Ras. Thalidomide inhibited the Ras activation induced by lipopolysaccharide (LPS) and vascular endothelial growth factor (VEGF) as well as EGF. There was no significant difference in the expression and function of EGF receptor between thalidomide-treated and non-treated cells. Therefore, thalidomide was suggested to inhibit EGF-induced cell growth via inactivation of Ras.
|
| Volume |
374(4)
|
| Pages |
683-7
|
| Published |
2008-10-3
|
| DOI |
10.1016/j.bbrc.2008.07.090
|
| PII |
S0006-291X(08)01432-0
|
| PMID |
18662673
|
| MeSH |
Angiogenesis Inhibitors / pharmacology*
Animals
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Cell Proliferation / drug effects*
Enzyme Activation
Epidermal Growth Factor / antagonists & inhibitors*
Epidermal Growth Factor / pharmacology
ErbB Receptors / metabolism
Humans
Leukemia / enzymology*
Mice
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / metabolism
Monocytes / drug effects
Monocytes / enzymology
Phosphorylation
Thalidomide / pharmacology*
Vascular Endothelial Growth Factor A / pharmacology
p38 Mitogen-Activated Protein Kinases / metabolism
ras Proteins / antagonists & inhibitors*
ras Proteins / metabolism
|
| IF |
2.985
|
| Times Cited |
8
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
THP-1(RCB1189)
RAW 264(RCB0535)
U-937 DE-4(RCB0435) |
