論文 - 詳細
| RRC ID | 43465 |
|---|---|
| 著者 | Inoue M, Matsumoto S, Saito H, Tsujitani S, Ikeguchi M. |
| タイトル | Intraperitoneal administration of a small interfering RNA targeting nuclear factor-kappa B with paclitaxel successfully prolongs the survival of xenograft model mice with peritoneal metastasis of gastric cancer. |
| ジャーナル | Int J Cancer |
| Abstract |
Activation of nuclear factor-kappa B (NF-kappaB) has been detected in various malignant tumors, including gastric carcinoma, and is associated with tumor growth, metastasis, resistance to chemotherapeutic agents and poor prognosis. Therefore, NF-kappaB is a potential target for antitumor therapy. In this study, we used a small interfering RNA (siRNA) to knockdown NF-kappaB p65 expression and determined whether intraperitoneal administration of NF-kappaB p65 siRNA and paclitaxel was effective for treating peritoneal metastasis of gastric cancer. Western blot analysis revealed that NF-kappaB p65 expression was diminished by NF-kappaB p65 siRNA. Apoptotic cells were increased after transfection of NF-kappaB p65 siRNA compared with control siRNA in the treatment with paclitaxel. In a murine xenograft model, abundant fluorescence was observed on the surface of intraperitoneal nodules of gastric cancer after siRNA administration. Moreover, intraperitoneal administration of NF-kappaB p65 siRNA reduced NF-kappaB expression in intraperitoneal nodules of gastric cancer. Finally, mice treated by intraperitoneal administration of NF-kappaB p65 siRNA and paclitaxel survived for a significantly longer time than mice treated by intraperitoneal administration of paclitaxel alone (p = 0.0002). Taken together, the present results demonstrate that intraperitoneal administration of NF-kappaB p65 siRNA and paclitaxel inhibited cancer growth in mice with peritoneal metastasis of gastric cancer. Therefore, intraperitoneal administration of NF-kappaB p65 siRNA and paclitaxel may provide a breakthrough in the treatment of peritoneal metastasis of gastric cancer. |
| 巻・号 | 123(11) |
| ページ | 2696-701 |
| 公開日 | 2008-12-1 |
| DOI | 10.1002/ijc.23867 |
| PMID | 18798274 |
| MeSH | Animals Cell Line, Tumor Combined Modality Therapy Female Genetic Therapy Injections, Intraperitoneal Mice Mice, Inbred BALB C Paclitaxel / administration & dosage Paclitaxel / therapeutic use* Peritoneal Neoplasms / genetics Peritoneal Neoplasms / metabolism* Peritoneal Neoplasms / secondary Peritoneal Neoplasms / therapy* RNA, Small Interfering / genetics* Stomach Neoplasms / genetics Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology Stomach Neoplasms / therapy* Survival Rate Time Factors Transcription Factor RelA / genetics Transcription Factor RelA / metabolism* Xenograft Model Antitumor Assays |
| IF | 5.145 |
| 引用数 | 18 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) MKN1(RCB1003) |