| RRC ID |
43486
|
| 著者 |
Ishiguro K, Ando T, Goto H.
|
| タイトル |
Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells.
|
| ジャーナル |
Biotechniques
|
| Abstract |
4-Nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ) reacts with carboxylic acids in the presence of condensing agents and is utilized for the fluorescence detection of the generated derivatives in high performance liquid chromatography or capillary electro-phoresis. Although the fluorescence intensity of derivatives of NBD-PZ-CH2CH2NH2 with carboxylic acids is elevated at low pH, the pH-dependent fluorescence of NBD-PZ itself has not yet been investigated. In this study, we determined the fluorescence spectra of NBD-PZ at various pH and found that the fluorescence intensity of NBD-PZ was elevated dramatically at pH
|
| 巻・号 |
45(4)
|
| ページ |
465, 467-8
|
| 公開日 |
2008-10-1
|
| DOI |
10.2144/000112912
|
| PII |
000112912
|
| PMID |
18855774
|
| MeSH |
Animals
Cell Line
Cell Survival
Cytological Techniques*
Humans
Lysosomes / metabolism*
Mice
Microscopy, Fluorescence
Oxadiazoles / chemistry
Oxadiazoles / economics
Oxadiazoles / metabolism*
Piperazines / chemistry
Piperazines / economics
Piperazines / metabolism*
Rats
|
| IF |
1.541
|
| 引用数 |
11
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HGC-27(RCB0500)
CW-2(RCB0778) |
