論文 - 詳細
| RRC ID | 43486 |
|---|---|
| 著者 | Ishiguro K, Ando T, Goto H. |
| タイトル | Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. |
| ジャーナル | Biotechniques |
| Abstract |
4-Nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ) reacts with carboxylic acids in the presence of condensing agents and is utilized for the fluorescence detection of the generated derivatives in high performance liquid chromatography or capillary electro-phoresis. Although the fluorescence intensity of derivatives of NBD-PZ-CH2CH2NH2 with carboxylic acids is elevated at low pH, the pH-dependent fluorescence of NBD-PZ itself has not yet been investigated. In this study, we determined the fluorescence spectra of NBD-PZ at various pH and found that the fluorescence intensity of NBD-PZ was elevated dramatically at pH |
| 巻・号 | 45(4) |
| ページ | 465, 467-8 |
| 公開日 | 2008-10-1 |
| DOI | 10.2144/000112912 |
| PII | 000112912 |
| PMID | 18855774 |
| MeSH | Animals Cell Line Cell Survival Cytological Techniques* Humans Lysosomes / metabolism* Mice Microscopy, Fluorescence Oxadiazoles / chemistry Oxadiazoles / economics Oxadiazoles / metabolism* Piperazines / chemistry Piperazines / economics Piperazines / metabolism* Rats |
| IF | 1.541 |
| 引用数 | 11 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HGC-27(RCB0500) CW-2(RCB0778) |