Reference - Detail
| RRC ID | 43486 |
|---|---|
| Author | Ishiguro K, Ando T, Goto H. |
| Title | Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. |
| Journal | Biotechniques |
| Abstract |
4-Nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ) reacts with carboxylic acids in the presence of condensing agents and is utilized for the fluorescence detection of the generated derivatives in high performance liquid chromatography or capillary electro-phoresis. Although the fluorescence intensity of derivatives of NBD-PZ-CH2CH2NH2 with carboxylic acids is elevated at low pH, the pH-dependent fluorescence of NBD-PZ itself has not yet been investigated. In this study, we determined the fluorescence spectra of NBD-PZ at various pH and found that the fluorescence intensity of NBD-PZ was elevated dramatically at pH |
| Volume | 45(4) |
| Pages | 465, 467-8 |
| Published | 2008-10-1 |
| DOI | 10.2144/000112912 |
| PII | 000112912 |
| PMID | 18855774 |
| MeSH | Animals Cell Line Cell Survival Cytological Techniques* Humans Lysosomes / metabolism* Mice Microscopy, Fluorescence Oxadiazoles / chemistry Oxadiazoles / economics Oxadiazoles / metabolism* Piperazines / chemistry Piperazines / economics Piperazines / metabolism* Rats |
| IF | 1.541 |
| Times Cited | 11 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HGC-27(RCB0500) CW-2(RCB0778) |