RRC ID 43486
著者 Ishiguro K, Ando T, Goto H.
タイトル Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells.
ジャーナル Biotechniques
Abstract 4-Nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ) reacts with carboxylic acids in the presence of condensing agents and is utilized for the fluorescence detection of the generated derivatives in high performance liquid chromatography or capillary electro-phoresis. Although the fluorescence intensity of derivatives of NBD-PZ-CH2CH2NH2 with carboxylic acids is elevated at low pH, the pH-dependent fluorescence of NBD-PZ itself has not yet been investigated. In this study, we determined the fluorescence spectra of NBD-PZ at various pH and found that the fluorescence intensity of NBD-PZ was elevated dramatically at pH
巻・号 45(4)
ページ 465, 467-8
公開日 2008-10-1
DOI 10.2144/000112912
PII 000112912
PMID 18855774
MeSH Animals Cell Line Cell Survival Cytological Techniques* Humans Lysosomes / metabolism* Mice Microscopy, Fluorescence Oxadiazoles / chemistry Oxadiazoles / economics Oxadiazoles / metabolism* Piperazines / chemistry Piperazines / economics Piperazines / metabolism* Rats
IF 1.541
引用数 11
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HGC-27(RCB0500) CW-2(RCB0778)