RRC ID 43522
著者 Niwa M, Nitta A, Cen X, Kitaichi K, Ozaki N, Yamada K, Nabeshima T.
タイトル A novel molecule 'shati' increases dopamine uptake via the induction of tumor necrosis factor-alpha in pheochromocytoma-12 cells.
ジャーナル J Neurochem
Abstract The psychostimulant properties of methamphetamine (METH) are associated with an increase in extracellular dopamine (DA) levels in the brain, via facilitation of DA's release from pre-synaptic nerve terminals and inhibition of its reuptake through DA transporter. Recently, we have demonstrated that tumor necrosis factor-alpha (TNF-alpha) increases DA uptake and inhibits METH dependence. Moreover, we have clarified 'shati' identified in the nucleus accumbens of mice treated with METH is involved in METH dependence. In the present study, we investigated the effects of TNF-alpha on DA uptake in PC12 cells and established a PC12 cell line transfected with a vector containing shati cDNA to examine the precise mechanism behind the role of shati in DA uptake. Moreover, we examined the relationship between shati and TNF-alpha. TNF-alpha increased DA uptake via the mitogen-activated protein kinase kinase pathway and inhibited the METH-induced decrease in DA uptake in PC12 cells. Transfection of the vector containing shati cDNA into PC12 cells, induced the expression of shati and TNF-alpha mRNA, accelerated DA uptake, and inhibited the METH-induced decrease in DA uptake. These results suggest that the functional roles of shati in METH-regulated behavioral changes are mediated through inhibition of the METH-induced decrease in DA uptake via TNF-alpha.
巻・号 107(6)
ページ 1697-708
公開日 2008-12-1
DOI 10.1111/j.1471-4159.2008.05738.x
PII JNC5738
PMID 19014384
MeSH Acetyltransferases Analysis of Variance Animals Dopamine / metabolism* Dopamine Uptake Inhibitors / pharmacology Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Gene Expression Regulation / drug effects Gene Expression Regulation / genetics Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Male Methamphetamine / pharmacology Mitogen-Activated Protein Kinase Kinases / metabolism PC12 Cells / drug effects* PC12 Cells / metabolism Rats Transfection Tritium / metabolism Tumor Necrosis Factor-alpha / metabolism Tumor Necrosis Factor-alpha / pharmacology* Tyrosine 3-Monooxygenase / metabolism
IF 4.066
引用数 13
WOS 分野 NEUROSCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009)