論文 - 詳細
| RRC ID | 43522 |
|---|---|
| 著者 | Niwa M, Nitta A, Cen X, Kitaichi K, Ozaki N, Yamada K, Nabeshima T. |
| タイトル | A novel molecule 'shati' increases dopamine uptake via the induction of tumor necrosis factor-alpha in pheochromocytoma-12 cells. |
| ジャーナル | J Neurochem |
| Abstract |
The psychostimulant properties of methamphetamine (METH) are associated with an increase in extracellular dopamine (DA) levels in the brain, via facilitation of DA's release from pre-synaptic nerve terminals and inhibition of its reuptake through DA transporter. Recently, we have demonstrated that tumor necrosis factor-alpha (TNF-alpha) increases DA uptake and inhibits METH dependence. Moreover, we have clarified 'shati' identified in the nucleus accumbens of mice treated with METH is involved in METH dependence. In the present study, we investigated the effects of TNF-alpha on DA uptake in PC12 cells and established a PC12 cell line transfected with a vector containing shati cDNA to examine the precise mechanism behind the role of shati in DA uptake. Moreover, we examined the relationship between shati and TNF-alpha. TNF-alpha increased DA uptake via the mitogen-activated protein kinase kinase pathway and inhibited the METH-induced decrease in DA uptake in PC12 cells. Transfection of the vector containing shati cDNA into PC12 cells, induced the expression of shati and TNF-alpha mRNA, accelerated DA uptake, and inhibited the METH-induced decrease in DA uptake. These results suggest that the functional roles of shati in METH-regulated behavioral changes are mediated through inhibition of the METH-induced decrease in DA uptake via TNF-alpha. |
| 巻・号 | 107(6) |
| ページ | 1697-708 |
| 公開日 | 2008-12-1 |
| DOI | 10.1111/j.1471-4159.2008.05738.x |
| PII | JNC5738 |
| PMID | 19014384 |
| MeSH | Acetyltransferases Analysis of Variance Animals Dopamine / metabolism* Dopamine Uptake Inhibitors / pharmacology Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Gene Expression Regulation / drug effects Gene Expression Regulation / genetics Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Male Methamphetamine / pharmacology Mitogen-Activated Protein Kinase Kinases / metabolism PC12 Cells / drug effects* PC12 Cells / metabolism Rats Transfection Tritium / metabolism Tumor Necrosis Factor-alpha / metabolism Tumor Necrosis Factor-alpha / pharmacology* Tyrosine 3-Monooxygenase / metabolism |
| IF | 4.066 |
| 引用数 | 13 |
| WOS 分野 | NEUROSCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |