| RRC ID |
43523
|
| Author |
Kuwabara Y, Yamada T, Yamazaki K, Du WL, Banno K, Aoki D, Sakamoto M.
|
| Title |
Establishment of an ovarian metastasis model and possible involvement of E-cadherin down-regulation in the metastasis.
|
| Journal |
Cancer Sci
|
| Abstract |
Clinical observations of cases of ovarian metastasis suggest that there may be a unique mechanism underlying ovarian-specific metastasis. This study was undertaken to establish an in vivo model of metastasis to the ovary, and to investigate the mechanism of ovarian-specific metastasis. We examined the capacity for ovarian metastasis in eight different human carcinoma cell lines by implantation in female NOD/SCID mice transvenously and intraperitoneally. By transvenous inoculation, only RERF-LC-AI, a poorly differentiated carcinoma cell line, frequently demonstrated ovarian metastasis. By intraperitoneal inoculation, four of the eight cell lines (HGC27, MKN-45, KATO-III, and RERF-LC-AI) metastasized to the ovary. We compared E-cadherin expression among ovarian metastatic cell lines and others. All of these four ovarian metastatic cell lines and HSKTC, a Krukenberg tumor cell line, showed E-cadherin down-regulation and others did not. E-cadherin was then forcibly expressed in RERF-LC-AI, and inhibited ovarian metastasis completely. The capacity for metastasizing to the other organs was not affected by E-cadherin expression. We also performed histological investigation of clinical ovarian-metastatic tumor cases. About half of all ovarian-metastatic tumor cases showed loss or reduction of E-cadherin expression. These data suggest that E-cadherin down-regulation may be involved in ovarian-specific metastasis.
|
| Volume |
99(10)
|
| Pages |
1933-9
|
| Published |
2008-10-1
|
| DOI |
10.1111/j.1349-7006.2008.00946.x
|
| PII |
CAS946
|
| PMID |
19016752
|
| MeSH |
Animals
Cadherins / genetics*
Cadherins / metabolism
Carcinoma / genetics
Carcinoma / metabolism
Carcinoma / pathology
Cell Line, Tumor
Disease Models, Animal*
Down-Regulation*
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Mice
Mice, Inbred NOD
Mice, SCID
Neoplasm Metastasis
Ovarian Neoplasms / genetics
Ovarian Neoplasms / metabolism*
Ovarian Neoplasms / pathology*
Xenograft Model Antitumor Assays
|
| IF |
4.966
|
| Times Cited |
20
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
MKN28(RCB1000)
MKN45(RCB1001)
MKN74(RCB1002)
Kato III(RCB2088)
HGC-27(RCB0500)
HSKTC(RCB0515)
RERF-LC-AI(RCB0444) |
