RRC ID 43587
著者 Mezaki Y, Morii M, Yoshikawa K, Yamaguchi N, Satoyoshi K, Miura M, Imai K, Hebiguchi T, Habuchi T, Senoo H.
タイトル Elevated expression of transforming growth factor β3 in carbon tetrachloride-treated rat liver and involvement of retinoid signaling.
ジャーナル Int J Mol Med
Abstract Transforming growth factor (TGF) β is a pro-fibrotic cytokine. While three isoforms (TGF-β1, 2 and 3) are known, the functional differences between them are obscure. To investigate the roles of TGF-β isoforms during liver fibrogenesis, male Wistar rats were administrated carbon tetrachloride (CCl4) subcutaneously twice a week for two months. Livers were excised and sectioned for histochemical examinations. These livers were also used to quantitate the expression of genes associated with fibrogenesis, including TGF-β isoforms, as well as those associated with retinoid metabolism. Expression levels of Tgfb1 and Tgfb3 were up-regulated in CCl4-treated rat livers while that of Tgfb2 was not changed. The mRNAs for lecithin-retinol acyltransferase (Lrat) and retinoic acid hydroxylase, Cyp26a1, were also elevated. By immunohistochemical staining, TGF-β3 protein was found to be localized mainly in liver parenchymal cells (hepatocytes). These results indicate that retinoid mobilization likely takes place within the rat's liver following CCl4 treatment, and suggest the possibility that the expression of Tgfb mRNA is regulated by retinoic acid receptors. Reporter analyses of a region of the Tgfb3 gene were performed using the rat liver parenchymal cell line, RLC-16, and a positively responsive region was identified within its intron.
巻・号 29(1)
ページ 18-24
公開日 2012-1-1
DOI 10.3892/ijmm.2011.809
PMID 21993772
MeSH Animals Carbon Tetrachloride Poisoning / genetics Carbon Tetrachloride Poisoning / metabolism* Carbon Tetrachloride Poisoning / pathology Cell Line Gene Expression Profiling Gene Expression Regulation Immunohistochemistry Liver / metabolism Liver / pathology Liver Cirrhosis, Experimental / chemically induced Liver Cirrhosis, Experimental / genetics Liver Cirrhosis, Experimental / metabolism* Liver Cirrhosis, Experimental / pathology Male Mice Polymerase Chain Reaction Protein Isoforms Rats Rats, Wistar Retinoids / genetics Retinoids / metabolism* Signal Transduction Transforming Growth Factor beta3 / biosynthesis* Transforming Growth Factor beta3 / genetics Transforming Growth Factor beta3 / metabolism
IF 3.098
引用数 4
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL
リソース情報
ヒト・動物細胞