RRC ID 43602
Author Takayama N, Eto K.
Title In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells.
Journal Methods Mol. Biol.
Abstract Human embryonic stem cells (hESCs) represent a potential source of blood cells for transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. Moreover, human-induced pluripotent stem cells (hiPSCs), recently established by defined reprogramming factors expressed in somatic cells, represent a further source for the generation of hematopoietic cells. When undifferentiated hESCs or hiPSCs are cultured on either mesenchymal C3H10T1/2 cells or OP-9 stromal cells, they can be differentiated into a hematopoietic niche that concentrates hematopoietic progenitors, which we named "embryonic stem cell-derived sacs" (ES-sacs). We have optimized the in vitro culture condition for obtaining mature megakaryocytes derived from the hematopoietic progenitors within ES-sacs, which are then able to release platelets. These in vitro-generated platelets display integrin activation capability, indicating normal hemostatic function. This novel protocol thus provides a means of generating platelets from hESCs as well as hiPSCs, for the study of normal human thrombopoiesis and also thrombopoiesis in disease conditions using patient-specific hiPSCs.
Volume 788
Pages 205-17
Published 2012
DOI 10.1007/978-1-61779-307-3_15
PMID 22130710
MeSH Animals Blood Platelets / cytology* Cells, Cultured Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Megakaryocytes / cytology* Mice Thrombopoiesis*
Times Cited 18
Human and Animal Cells