Reference - Detail
| RRC ID | 43602 |
|---|---|
| Author | Takayama N, Eto K. |
| Title | In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells. |
| Journal | Methods Mol Biol |
| Abstract |
Human embryonic stem cells (hESCs) represent a potential source of blood cells for transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. Moreover, human-induced pluripotent stem cells (hiPSCs), recently established by defined reprogramming factors expressed in somatic cells, represent a further source for the generation of hematopoietic cells. When undifferentiated hESCs or hiPSCs are cultured on either mesenchymal C3H10T1/2 cells or OP-9 stromal cells, they can be differentiated into a hematopoietic niche that concentrates hematopoietic progenitors, which we named "embryonic stem cell-derived sacs" (ES-sacs). We have optimized the in vitro culture condition for obtaining mature megakaryocytes derived from the hematopoietic progenitors within ES-sacs, which are then able to release platelets. These in vitro-generated platelets display integrin activation capability, indicating normal hemostatic function. This novel protocol thus provides a means of generating platelets from hESCs as well as hiPSCs, for the study of normal human thrombopoiesis and also thrombopoiesis in disease conditions using patient-specific hiPSCs. |
| Volume | 788 |
| Pages | 205-17 |
| Published | 2012-1-1 |
| DOI | 10.1007/978-1-61779-307-3_15 |
| PMID | 22130710 |
| MeSH | Animals Blood Platelets / cytology* Cells, Cultured Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Megakaryocytes / cytology* Mice Thrombopoiesis* |
| Times Cited | 30 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 10T1/2(RCB0247) |