論文 - 詳細
| RRC ID | 43602 |
|---|---|
| 著者 | Takayama N, Eto K. |
| タイトル | In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Human embryonic stem cells (hESCs) represent a potential source of blood cells for transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. Moreover, human-induced pluripotent stem cells (hiPSCs), recently established by defined reprogramming factors expressed in somatic cells, represent a further source for the generation of hematopoietic cells. When undifferentiated hESCs or hiPSCs are cultured on either mesenchymal C3H10T1/2 cells or OP-9 stromal cells, they can be differentiated into a hematopoietic niche that concentrates hematopoietic progenitors, which we named "embryonic stem cell-derived sacs" (ES-sacs). We have optimized the in vitro culture condition for obtaining mature megakaryocytes derived from the hematopoietic progenitors within ES-sacs, which are then able to release platelets. These in vitro-generated platelets display integrin activation capability, indicating normal hemostatic function. This novel protocol thus provides a means of generating platelets from hESCs as well as hiPSCs, for the study of normal human thrombopoiesis and also thrombopoiesis in disease conditions using patient-specific hiPSCs. |
| 巻・号 | 788 |
| ページ | 205-17 |
| 公開日 | 2012-1-1 |
| DOI | 10.1007/978-1-61779-307-3_15 |
| PMID | 22130710 |
| MeSH | Animals Blood Platelets / cytology* Cells, Cultured Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Megakaryocytes / cytology* Mice Thrombopoiesis* |
| 引用数 | 30 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |