論文 - 詳細
| RRC ID | 43606 |
|---|---|
| 著者 | Sasakawa Y, Kominami A, Yamamoto K, Nakaoka F, Nakamura M, Nakao M, Abe M, Fukuhama C, Kagawa K. |
| タイトル | Effects of globin digest and its active ingredient Trp-Thr-Gln-Arg on galactosamine/lipopolysaccharide-induced liver injury in ICR mice. |
| ジャーナル | Life Sci |
| Abstract |
AIMS:We investigated the effects of globin digest (GD) and its active ingredient Trp-Thr-Gln-Arg (WTQR) on galactosamine/lipopolysaccharide (GalN/LPS)-induced liver injury in imprinting control region (ICR) mice. MAIN METHODS:The effects of WTQR and GD on the liver injury were examined by measuring the survival rate, serum aminotransferase activities, hepatic components, antioxidant enzyme activities, histopathological analysis, serum levels and hepatic gene expression of tumor necrosis factor-alpha (TNF-α), macrophage inflammatory protein-2 (MIP-2), and nitric oxide (NO) or inducible nitric oxide synthase (iNOS), and nuclear factor-kappa B (NF-κB) p65 content in GalN/LPS-treated ICR mice. RAW264 mouse macrophages were used to confirm the anti-inflammatory effects of WTQR and GD on the macrophages. KEY FINDINGS:WTQR and GD increased the survival rate, suppressed the serum aminotransferase activities, serum levels and hepatic gene expression of TNF-α, MIP-2, and NO or iNOS, and nuclear NF-κB p65 content in GalN/LPS-treated mice; decreased the oxidized glutathione content, increased the superoxide dismutase activity, and decreased the histopathological grade values of the hepatocyte necrosis and lobular inflammation in GalN/LPS-injured liver; and suppressed the release levels and gene expression of TNF-α, MIP-2, and NO or iNOS, and nuclear NF-κB p65 content in LPS-stimulated RAW264 macrophages. WTQR and GD may improve the antioxidant defense system and inflammatory status in GalN/LPS-injured liver. SIGNIFICANCE:These findings indicate that WTQR and GD have hepatoprotective effects on GalN/LPS-induced liver injury in ICR mice. |
| 巻・号 | 90(5-6) |
| ページ | 190-9 |
| 公開日 | 2012-1-30 |
| DOI | 10.1016/j.lfs.2011.11.013 |
| PII | S0024-3205(11)00575-3 |
| PMID | 22154906 |
| MeSH | Analysis of Variance Animals Antioxidants / metabolism Cell Line Chemical and Drug Induced Liver Injury / drug therapy* Chemical and Drug Induced Liver Injury / immunology Chemical and Drug Induced Liver Injury / metabolism Chemokine CXCL2 / blood Chemokine CXCL2 / genetics Galactosamine / administration & dosage Galactosamine / adverse effects Globins / administration & dosage* Globins / chemistry Hepatocytes / drug effects Hepatocytes / metabolism Lipopolysaccharides / administration & dosage Lipopolysaccharides / adverse effects Liver / drug effects Liver / metabolism Liver / pathology Macrophages / metabolism Male Mice Mice, Inbred ICR NF-kappa B / analysis NF-kappa B / metabolism Nitric Oxide / blood Nitric Oxide / metabolism Nitric Oxide Synthase Type II / genetics Nitric Oxide Synthase Type II / metabolism Oligopeptides / administration & dosage* Peptide Fragments / administration & dosage* Peptide Fragments / chemistry Survival Rate Transaminases / blood Tumor Necrosis Factor-alpha / blood Tumor Necrosis Factor-alpha / genetics |
| IF | 3.647 |
| 引用数 | 5 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |