RRC ID 43638
Author Yagi S, Matsuda M, Kiyokawa E.
Title Suppression of Rac1 activity at the apical membrane of MDCK cells is essential for cyst structure maintenance.
Journal EMBO Rep.
Abstract Using MDCK cells that constitutively express a Förster resonance energy transfer biosensor, we found that Rac1 activity is homogenous at the entire plasma membrane in early stages of cystogenesis, whereas in later stages Rac1 activity is higher at the lateral membrane than at the apical plasma membrane. If Rac1 is activated at the apical membrane in later stages, however, the monolayer cells move into the luminal space. In these cells, tight junctions are disrupted, accompanied by mislocalization of polarization markers and disorientation of cell division. These observations indicate that Rac1 suppression at the apical membrane is essential for the maintenance of cyst structure.
Volume 13(3)
Pages 237-43
Published 2012-3-1
DOI 10.1038/embor.2011.249
PII embor2011249
PMID 22261715
PMC PMC3323125
MeSH Animals Cell Line Cell Membrane / metabolism* Cell Polarity* Cysts / metabolism* Dogs Epithelium / metabolism Tight Junctions / metabolism rac1 GTP-Binding Protein / genetics rac1 GTP-Binding Protein / metabolism*
IF 8.749
Times Cited 16
Human and Animal Cells