論文 - 詳細
| RRC ID | 43705 |
|---|---|
| 著者 | Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H. |
| タイトル | Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Smads are intracellular signaling mediators. Complexes of Smad2 and Smad3 with Smad4 transmit transforming growth factor-beta (TGF-β) receptor-induced signaling. Snail plays important roles in mesoderm formation, gastrulation, neural crest development, and epithelial mesenchymal transition. However, it remains unknown whether Smad3 and Snail expression is circadian rhythm-dependent. Here, we showed for the first time that Smad3 and Snail show circadian expression in human gingival fibroblasts (HGF-1) and human mesenchymal stem cells (MSC) after serum shock. They also showed circadian expression in the mouse liver. We confirmed that BMAL1/2, DEC1/2, VEGF, and PER1/2/3 also show circadian expression in both HGF-1 and MSC. The mRNA peaks and phases in circadian expression of these genes differed between HGF-1 and MSC. In a luciferase assay, Smad3 promoter activity was upregulated by CLOCK/BMAL1. These findings suggest that Smad3 and Snail have circadian rhythm in vitro and vivo, and that circadian expression of Smad3 depends on CLOCK/BMAL1. |
| 巻・号 | 419(2) |
| ページ | 441-6 |
| 公開日 | 2012-3-9 |
| DOI | 10.1016/j.bbrc.2012.02.076 |
| PII | S0006-291X(12)00309-9 |
| PMID | 22382019 |
| MeSH | ARNTL Transcription Factors / biosynthesis Animals Basic Helix-Loop-Helix Transcription Factors / biosynthesis CLOCK Proteins / metabolism Cells, Cultured Circadian Rhythm* Fibroblasts / metabolism* Gingiva / metabolism* Humans Liver / metabolism* Male Mesenchymal Stem Cells / metabolism* Mice Period Circadian Proteins / biosynthesis Smad3 Protein / biosynthesis* Snail Family Transcription Factors Transcription Factors / biosynthesis* Tumor Suppressor Proteins / biosynthesis Vascular Endothelial Growth Factor A / biosynthesis |
| IF | 2.985 |
| 引用数 | 17 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MSC-R37(MSC; MSC-R37) |