RRC ID |
43705
|
著者 |
Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H.
|
タイトル |
Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Smads are intracellular signaling mediators. Complexes of Smad2 and Smad3 with Smad4 transmit transforming growth factor-beta (TGF-β) receptor-induced signaling. Snail plays important roles in mesoderm formation, gastrulation, neural crest development, and epithelial mesenchymal transition. However, it remains unknown whether Smad3 and Snail expression is circadian rhythm-dependent. Here, we showed for the first time that Smad3 and Snail show circadian expression in human gingival fibroblasts (HGF-1) and human mesenchymal stem cells (MSC) after serum shock. They also showed circadian expression in the mouse liver. We confirmed that BMAL1/2, DEC1/2, VEGF, and PER1/2/3 also show circadian expression in both HGF-1 and MSC. The mRNA peaks and phases in circadian expression of these genes differed between HGF-1 and MSC. In a luciferase assay, Smad3 promoter activity was upregulated by CLOCK/BMAL1. These findings suggest that Smad3 and Snail have circadian rhythm in vitro and vivo, and that circadian expression of Smad3 depends on CLOCK/BMAL1.
|
巻・号 |
419(2)
|
ページ |
441-6
|
公開日 |
2012-3-9
|
DOI |
10.1016/j.bbrc.2012.02.076
|
PII |
S0006-291X(12)00309-9
|
PMID |
22382019
|
MeSH |
ARNTL Transcription Factors / biosynthesis
Animals
Basic Helix-Loop-Helix Transcription Factors / biosynthesis
CLOCK Proteins / metabolism
Cells, Cultured
Circadian Rhythm*
Fibroblasts / metabolism*
Gingiva / metabolism*
Humans
Liver / metabolism*
Male
Mesenchymal Stem Cells / metabolism*
Mice
Period Circadian Proteins / biosynthesis
Smad3 Protein / biosynthesis*
Snail Family Transcription Factors
Transcription Factors / biosynthesis*
Tumor Suppressor Proteins / biosynthesis
Vascular Endothelial Growth Factor A / biosynthesis
|
IF |
2.985
|
引用数 |
17
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MSC-R37(MSC; MSC-R37) |