RRC ID 43705
著者 Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H.
タイトル Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver.
ジャーナル Biochem Biophys Res Commun
Abstract Smads are intracellular signaling mediators. Complexes of Smad2 and Smad3 with Smad4 transmit transforming growth factor-beta (TGF-β) receptor-induced signaling. Snail plays important roles in mesoderm formation, gastrulation, neural crest development, and epithelial mesenchymal transition. However, it remains unknown whether Smad3 and Snail expression is circadian rhythm-dependent. Here, we showed for the first time that Smad3 and Snail show circadian expression in human gingival fibroblasts (HGF-1) and human mesenchymal stem cells (MSC) after serum shock. They also showed circadian expression in the mouse liver. We confirmed that BMAL1/2, DEC1/2, VEGF, and PER1/2/3 also show circadian expression in both HGF-1 and MSC. The mRNA peaks and phases in circadian expression of these genes differed between HGF-1 and MSC. In a luciferase assay, Smad3 promoter activity was upregulated by CLOCK/BMAL1. These findings suggest that Smad3 and Snail have circadian rhythm in vitro and vivo, and that circadian expression of Smad3 depends on CLOCK/BMAL1.
巻・号 419(2)
ページ 441-6
公開日 2012-3-9
DOI 10.1016/j.bbrc.2012.02.076
PII S0006-291X(12)00309-9
PMID 22382019
MeSH ARNTL Transcription Factors / biosynthesis Animals Basic Helix-Loop-Helix Transcription Factors / biosynthesis CLOCK Proteins / metabolism Cells, Cultured Circadian Rhythm* Fibroblasts / metabolism* Gingiva / metabolism* Humans Liver / metabolism* Male Mesenchymal Stem Cells / metabolism* Mice Period Circadian Proteins / biosynthesis Smad3 Protein / biosynthesis* Snail Family Transcription Factors Transcription Factors / biosynthesis* Tumor Suppressor Proteins / biosynthesis Vascular Endothelial Growth Factor A / biosynthesis
IF 2.985
引用数 17
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MSC-R37(MSC; MSC-R37)